Mad (Ne-Yo song)

"Mad" is a song by American pop/R&B singer Ne-Yo. It is the third single from his album Year of the Gentleman and was produced by Stargate and himself.

Music video

The video for the song was directed by Diane Martel. It premiered on AOL on November 25, 2008. The video, shot in black and white, presents a narrative wherein Ne-Yo argues with his girlfriend (played by Faune Chambers). After leaving, in an attempt to save a child from a car accident, someone is fatally hit. At the end of the video, it is revealed that the whole time it has been the ghost of Ne-Yo's character singing, and that it was he who was killed. The video to this song is Part 2 to his song Part of the List.

The music video appears to mirror the 1999 American thriller The Sixth Sense from M. Night Shyamalan, in which Bruce Willis plays a psychologist who is shot by an estranged patient of his. Based on how the film was shot and portrayed, Bruce Willis is shown in many situations as if he is there physically, when in actuality he was murdered by his patient, just as how Ne-Yo appears with his girlfriend, seeming to be there physically and that she is ignoring him due to their earlier argument, when he is actually dead.

Mad1

Mad1 is a non-essential protein in yeast which has a function in the spindle assembly checkpoint (SAC). This checkpoint monitors chromosome attachment to spindle microtubules and prevents cells from starting anaphase until the spindle is built up. The name Mad refers to the observation that mutant cells are mitotic arrest deficient (MAD) during microtubule depolymerization. Mad1 recruits the anaphase inhibitor Mad2 to unattached kinetochores and is essential for Mad2-Cdc20 complex formation in vivo but not in vitro. In vivo, Mad1 acts as a competitive inhibitor of the Mad2-Cdc20 complex. Mad1 is phosphorylated by Mps1 which then leads together with other activities to the formation of the mitotic checkpoint complex (MCC). Thereby it inhibits the activity of the anaphase-promoting complex/cyclosome (APC/C). Homolog’s of Mad1 are conserved in eukaryotes from yeast to mammals.

Introduction

In the early 90s, yeast genes were identified which mutations resulted in a defect in mitotic arrest in response to microtubule disassembly (mitotic arrest deficient genes - MAD genes). This cells showed during division no mitotic arrest in the presence of microtubule polymerization inhibitors and were therefore not able to delay cell division. The genes identified included the MAD1, MAD2 and MAD3 genes. They are conserved in all eukaryotes and are involved in a pathway that is active in prometaphase to prevent the premature separation of sister chromatids and constitute the so-called spindle assembly checkpoint(SAC). This checkpoint monitors the status of chromosome attachment to the mitotic spindle and inhibits the metaphase to anaphase transition by preventing the activation of the anaphase-promoting complex/cyclosome (APC/C), and thereby the degradation of cell cycle regulators. Mad1 is in this pathway accumulated at unattached kinetochores and acts as a sensor for unattached kinetochores in this machinery.

Mad2

Mad2 (mitotic arrest deficient 2) is an essential spindle checkpoint protein. The spindle checkpoint system is a regulatory system that restrains progression through the metaphase-to-anaphase transition. The Mad2 gene was first identified in the yeast S. cerevisiae in a screen for genes which when mutated would confer sensitivity to microtubule poisons. The human orthologues of Mad2 (MAD2L1 and MAD2L2) were first cloned in a search for human cDNAs that would rescue the microtubule poison-sensitivity of a yeast strain in which a kinetochore binding protein was missing. The protein was shown to be present at unattached kinetochores and antibody inhibition studies demonstrated it was essential to execute a block in the metaphase-to-anaphase transition in response to the microtubule poison nocodazole. Subsequent cloning of the Xenopus laevis orthologue, facilitated by the sharing of the human sequence, allowed for the characterization of the mitotic checkpoint in egg extracts.

Metaphase-to-anaphase transition

Angel (company)

Angel.com Incorporated was an American telecommunications company supplying interactive voice response, call center technology, and voice applications to businesses over the internet using the software as a service model. Angel was acquired in 2013 by Genesys and rebranded as Genesys Cloud.

History

Angel was developed by the CEO of MicroStrategy Michael J. Saylor, in 1997 as DSS Telecaster and DSS Broadcaster and were then merged into Angel.com. Moving away from the original telecaster idea, the company signed its first small business customers at the end of 2001 as a provider of hosted interactive voice response.

Angel.com was incorporated on April 30, 2008. In 2009, Dave Rennyson, former VP of Sales at Angel, was named President and COO replacing long-time CEO Michael Zirngibl. In 2011, Angel introduced Voice for Twitter Voice for Facebook, and Voice for Chatter, applications that add voice capabilities to the popular social networks and Salesforce.com's internal social network, Chatter.

Angel (Angel album)

Angel is the first album by the rock band Angel. "Tower", the keyboard-heavy opening track, was used widely during the late 1970s and early 1980s by album rock radio stations in the USA for various advertising purposes. The track is also on K-SHE radio's Classic List. This album can be seen as representing the band's early progressive roots, with Helluva Band seeing the group starting to move towards an increasingly hard rock-oriented sound. Tracks 6-8 segue to form a 10-minute mini suite.

Track listing

Personnel

References

Angel (season 5)

The fifth and final season of the television series Angel, the spin-off of Buffy the Vampire Slayer, premiered on October 1, 2003 on The WB and concluded its 22-episode season and its television run on May 19, 2004. The season aired on Wednesdays at 9:00 pm ET. This was the first and only season of Angel to air following the finale of Buffy the Vampire Slayer.

Season synopsis