MTT assay

The MTT assay is a colorimetric assay for assessing cell metabolic activity. NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present. These enzymes are capable of reducing the tetrazolium dye MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to its insoluble formazan, which has a purple color. Other closely related tetrazolium dyes including XTT, MTS and the WSTs, are used in conjunction with the intermediate electron acceptor, 1-methoxy phenazine methosulfate (PMS). With WST-1, which is cell-impermeable, reduction occurs outside the cell via plasma membrane electron transport. Tetrazolium dye assays can also be used to measure cytotoxicity (loss of viable cells) or cytostatic activity (shift from proliferation to quiescence) of potential medicinal agents and toxic materials. MTT assays are usually done in the dark since the MTT reagent is sensitive to light.

MTT and related tetrazolium salts

MTT, a yellow tetrazole, is reduced to purple formazan in living cells. A solubilization solution (usually either dimethyl sulfoxide, an acidified ethanol solution, or a solution of the detergent sodium dodecyl sulfate in diluted hydrochloric acid) is added to dissolve the insoluble purple formazan product into a colored solution. The absorbance of this colored solution can be quantified by measuring at a certain wavelength (usually between 500 and 600 nm) by a spectrophotometer. The degree of light absorption depends on the solvent.

.m2ts

M2TS is a filename extension used for the Blu-ray Disc Audio-Video (BDAV) MPEG-2 Transport Stream (M2TS) container file format. It is used for multiplexing audio, video and other streams. It is based on the MPEG-2 transport stream container. This container format is commonly used for high definition video on Blu-ray Disc and AVCHD.

Overview

The BDAV container format is a modification of MPEG-2 transport stream (ITU-T H.222.0 | ISO/IEC 13818-1) specification for random-access media, such as Blu-ray Disc, DVD, hard drives or solid-state memory cards. It is informally called M2TS.

In order to optimize the storage size, the format uses variable rate (VBR) Transport Streams instead of the typical constant rates (CBR) found in MPEG-2 TS broadcast. To be able to reconstruct a T-STD compliant constant rate Transport Stream for playback the arrival timestamp (ATS) of each packet needs to be recorded.

The standard MPEG-2 TS 188-byte packet is prefixed with a 4-byte extra header to a total size of 192 bytes. The header consists of a 2-bit copy permission indicator and the 30-bit arrival timestamp with a resolution of 27 MHz.

MTS

MTS or Mts may refer to:

Business

Computing

Education

Medicine and biology

Organizations and enterprises

MTs255

The MTs255 (Russian: МЦ255) is a shotgun fed by a 5-round internal revolving cylinder. It is produced by the TsKIB SOO Central Design and Research Bureau of Sporting and Hunting Arms. The Russian-made guns are available in 12, 20, 28 and 32 gauges, and .410 bore.

The MTs255 is unique in that the forearm extends nearly all the way back to the cylinder. The shotgun is reloaded in a manner consistent with that of most modern revolvers, by unlocking the cylinder, and swinging it away from the frame to the left and down.

See also

External links

Media related to MTs-255 at Wikimedia Commons