Jamie Ainscough (born 1972 in Sydney ), is an Australian former professional rugby league footballer of the 1990s and 2000s. An Australian international and New South Wales State of Origin representative three-quarter back, he played his club football for the Western Suburbs Magpies, Newcastle Knights, St. George Illawarra Dragons and Wigan Warriors (with whom he won the 2002 Challenge Cup).
Ainscough played for the Macquarie Cobras J.R.L.F.C in Cartwright, New South Wales. While attending Elderslie High School, Ainscough played for the Australian Schoolboys team in 1989. The following year he was playing first grade in the New South Wales Rugby League premiership for the Western Suburbs Magpies.
Ainscough also played 87 games with the Newcastle Knights from 1992 to 1996. He made one international appearance for Australia against New Zealand in the 3rd Test of 1995 Brisbane. In that game Ainscough came on as a replacement and scored a try.
Ainscough represented the New South Wales Rugby League team in the State of Origin series in 12 games between 1996 and 2001 (three as a replacement).