Growth factor receptor-bound protein 2 also known as Grb2 is an adaptor protein involved in signal transduction/cell communication. In humans, the GRB2 protein is encoded by the GRB2 gene.
The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Grb2 is widely expressed and is essential for multiple cellular functions. Inhibition of Grb2 function impairs developmental processes in various organisms and blocks transformation and proliferation of various cell types. It is thus not surprising that targeted gene disruption of Grb2 in mice is lethal at an early embryonic stage. Grb2 is best known for its ability to link the epidermal growth factor receptor tyrosine kinase to the activation of Ras and its downstream kinases, ERK1,2. Grb2 is composed of an SH2 domain flanked on each side by an SH3 domain. Grb2 has two closely related proteins with similar domain organizations, Gads and Grap. Gads and Grap are expressed specifically in hematopoietic cells and function in the coordination of tyrosine kinase mediated signal transduction.
What's happenin'?
What you're cryin' for?
You know you look too damn good to be cryin'
Come here
Do do do do do yeah yeah)
How about a player
DPG style
You know
You know
When I think of our untimely end
And everything we could have been
I cry, baby I cry
So it's 5 AM and
I still can't sleep
Took some medicine
But it's not working
Someone's clinging to me
And it's bittersweet
'Cause he's head over heels
But it ain't that deep
I finally changed my number
Got a different pager
Then last saturday
Your cousin called to say you're
Tryin' to reach me
Probably because you see that
I'm with someone new
Late at night like a little child
Wandering 'round alone
In my new friend's home
On my tippy toes
So that he won't know
(So that he won't know)
I still cry baby
(I cry baby)
Over you and me
Sipping Bailey's Cream
By the stereo
Trying to find relief
On the radio
I'm suppressing the tears
But they start to flow
'Cause the next song I hear
Is a song I wrote
When we first got together
Early that september
I can't bear to listen
So I might as well drift
In the kitchen
Pour another glass or two
And I try to forget you
(You)
Late at night like a little child
Wandering 'round alone
In my new friend's home
(In my new friend's home on my)
On my tippy toes
So that he won't know
(So that he won't know I)
I still cry baby
Over you and me
(You and me)
Baby hear me when I say
Let that man be on his way
Don't even waste your time
And worry how he handle thangz
(That ain't right) baby hear me when I say
A playa feelin' good today (u know ur 2 fly 2 be cryin')
Don't even waste your time watch how I handle (watch) thangz
Man ur a fool for this
For this)
But on the real dog I'm cool with this we do and this
D-O-DOUBLE G-Y
Kiss the girls and make them cry!
(It's late)
Late at night like a little child
(I'm like a little child)
Wandering around alone
(Wandering around alone)
In my new friend's home
(Do do do do do)
On my tippy toes
(Tippy toes)
So that he won't know
(So that he won't know)
I still cry baby
(I was babbling)
Over you and me
(Over you and me)
Why is it 5 AM and
I'm still struggeling and
Walking 'round no
Do do do do do do do
(On my tippy toes)
On my tippy toes
So that he won't know
I still cry baby
(I still cry)
Over you and me
I don't get no sleep
I'm half awake
Can't stop thinking of you and me
And everything we used to be
It could have been so perfectly
I cry
I cry
I cry
I gotta give me some sleep
Baby and I
On my tippy toes
So that he won't know
I still cry
You and I
Over you and me
Late at night like a little child
(Oh like a little child)
Wandering 'round alone
In my new friend's home
(Do do do do do)
(In my new friend's home)
On my tippy toes
So that he won't know
(So that he won't know)
I still cry
(Do do do do do)
(Do do do do do)
(Do do do do do)
(Do do do do do)
(Do do do do do)
Late at night like a little child
Wandering around alone
In my new friend's home
On my tippy toes
So that he won't know
I still cry baby
Over you and me
Do do do do do
Do do do do do
Do do do do do
Do do do do do
Cry baby
On my tippy toes
So that he won't know
I still cry
Mmmh