Flim may refer to:
Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography.
The lifetime of the fluorophore signal, rather than its intensity, is used to create the image in FLIM. This has the advantage of minimizing the effect of photon scattering in thick layers of sample.
A fluorophore which is excited by a photon will drop to the ground state with a certain probability based on the decay rates through a number of different (radiative and/or nonradiative) decay pathways. To observe fluorescence, one of these pathways must be by spontaneous emission of a photon. In the ensemble description, the fluorescence emitted will decay with time according to
where
In the above, is time,
is the fluorescence lifetime,
is the initial fluorescence at
, and
are the rates for each decay pathway, at least one of which must be the fluorescence decay rate
. More importantly, the lifetime,
is independent of the initial intensity and of the emitted light. This can be utilized for making non-intensity based measurements in chemical sensing.
Love will be the armor
Protect us from the pain
In our life
In our life
Even when the storm comes,
We'll stand out in the rain,
Watch it slide, live inside.
Here we stand against the world
You and I we rock the lullaby
Make me feel alive, cause the love will never day
Me put you long one,
Shot me down and we're gone,
Ain't no need to worry, don't call 911.
Bang-bang, a lot of bang-bang,
Shoot me with your love and got me feeling no pain.
Kill it, kill it baby with your love even, bang bang,
Baby, do it again.
So shoot me with your bullet, bullet, bullet,
Such a pretty bullet, bullet, bullet,
Go ahead and pull it, pull it, pull it,
Shoot me with your bullet, bullet, bullet.
So shoot me with your bullet, bullet, bullet,
Such a pretty bullet, bullet, bullet,
Go ahead and pull it, pull it, pull it,
Shoot me with your bullet, bullet, bullet.
Even if you're mad at me
And fighting all the time
It's alright, it's alright.
I would risk my sanity
I would lose my mind
For one night, for one night.
Here we stand against the world
You and I we rock the lullaby
Make me feel alive, cause the love will never day
Me put you long one,
Shot me down and we're gone,
Ain't no need to worry, don't call 911.
Bang-bang, a lot of bang-bang,
Shoot me with your love and got me feeling no pain.
Kill it, kill it baby with your love even, bang bang,
Baby, do it again.
So shoot me with your bullet, bullet, bullet,
Such a pretty bullet, bullet, bullet,
Go ahead and pull it, pull it, pull it,
Shoot me with your bullet, bullet, bullet.
So shoot me with your bullet, bullet, bullet,
Such a pretty bullet, bullet, bullet,
Go ahead and pull it, pull it, pull it,