FACT (biology)

FACT (facilitates chromatin transcription) is a heterodimeric protein complex that affects eukaryotic RNA polymerase II (Pol II) transcription elongation both in vitro and in vivo. It was discovered in 1998 as a factor purified from human cells that was essential for productive in vitro Pol II transcription on a chromatinized DNA template.

FACT consists of 140 and 80 kilodalton (kDa) subunits. The 140 kDa subunit is encoded by a human gene (SUPT16H) which is 36% identical to the S. cerevisiae gene Spt16 and the 80 kDa subunit is human SSRP1 (POB3 in S. cerevisiae). Both of these subunits in yeast affect Pol II transcription elongation, and purified human FACT binds specifically to mononucleosomes and the histone H2A/H2B dimer, but not to the H3/H4 tetramer (see: Nucleosome core particle) or Pol II.

Co-immunoprecipitation assays with tagged recombinant proteins showed that the Spt16 subunit interacts with H2A/H2B dimers and mononucleosomes, but not H3/H4 tetramers, whereas the SSRP1 subunit interacts only with H3/H4 tetramers and not mononucleosomes. Deletion of the highly acidic carboxy-terminus of Spt16 (a common feature of known histone chaperones) does not prevent Spt16 from forming a stable complex with SSRP1, but it does eliminate interaction with mononucleosomes and ability to stimulate in vitro transcription on chromatinized templates. The two subunits together, but neither alone, can stimulate formation of nucleosomes from free histones and DNA (histone chaperone activity). These two subunits are highly conserved across all eukaryotes, and in addition to transcription, have been shown to affect DNA repair and replication as well.

Biology

Biology is a natural science concerned with the study of life and living organisms, including their structure, function, growth, evolution, distribution, and taxonomy. Modern biology is a vast and eclectic field, composed of many branches and subdisciplines. However, despite the broad scope of biology, there are certain general and unifying concepts within it that govern all study and research, consolidating it into single, coherent fields. In general, biology recognizes the cell as the basic unit of life, genes as the basic unit of heredity, and evolution as the engine that propels the synthesis and creation of new species. It is also understood today that all organisms survive by consuming and transforming energy and by regulating their internal environment to maintain a stable and vital condition.

Subdisciplines of biology are defined by the scale at which organisms are studied, the kinds of organisms studied, and the methods used to study them: biochemistry examines the rudimentary chemistry of life; molecular biology studies the complex interactions among biological molecules; botany studies the biology of plants; cellular biology examines the basic building-block of all life, the cell; physiology examines the physical and chemical functions of tissues, organs, and organ systems of an organism; evolutionary biology examines the processes that produced the diversity of life; and ecology examines how organisms interact in their environment.

Biology (journal)

Biology is a quarterly, peer-reviewed, open access, scientific journal covering research on all aspects of biology. It was established in 2012 and is published by MDPI. The editor-in-chief is Chris O'Callaghan (University of Oxford). The journal publishes reviews, research papers, and communications.

Subject Areas

This journal covers all topics related to biology. More detail about the journal and its scope is available in the first editorial. Research fields of interest include, but are not limited to:

Biology: The Unity and Diversity of Life

Biology: The Unity and Diversity of Life is a college-level introductory biology textbook that covers basic biochemistry, anatomy, taxonomy, evolution, biotechnology and ecology. The book is designed to create in students a broad foundation of knowledge in biology and is frequently used in AP Biology classes in American high schools. Each book includes an interactive CD-ROM with links to additional instructional material. Like many textbooks new versions are printed every few years and reflect new gains in scientific knowledge. The book is published by Brooks/Cole and is currently in its twelfth edition. It was primarily compiled by Cecie Starr and Ralph Taggart with pictures and illustrations by Lisa Starr.

The book was translated into few languages, among others into Hebrew, published by the Open University of Israel.

External links

Fact (band)

Fact was a Japanese rock band, formed in December 1999 in Ibaraki Prefecture.

Clean and screamed singing styles are both used, and gang vocals are present in many choruses. Even though a Japanese band, the lyrics to most of the band's songs are written in English.

The members have hidden their faces during every video since 2009 wearing traditional Japanese Noh masks during the time they supported their second album, Fact (2009), but abandoned the imagery in videos the next year in favor of either partially or fully concealing their faces.

They are signed to Maximum10, an indie-rock imprint of the Avex Group.

History

The band was formed from members of other bands in the Tokyo area. They then began to tour the local music scene in and around Chiba, Mito and Tokyo. Originally, Tomohiro played bass and sang with support from Kazuki and Takahiro but, in 2002, main vocal duties as well as a third guitar were added when Hironobu joined. Hironobu had been a member of the band Second Base along with Kazuki before either of them joined Fact.

Data warehouse

In computing, a data warehouse (DW or DWH), also known as an enterprise data warehouse (EDW), is a system used for reporting and data analysis. DWs are central repositories of integrated data from one or more disparate sources. They store current and historical data and are used for creating analytical reports for knowledge workers throughout the enterprise. Examples of reports could range from annual and quarterly comparisons and trends to detailed daily sales analyses.

The data stored in the warehouse is uploaded from the operational systems (such as marketing, sales, etc., shown in the figure to the right). The data may pass through an operational data store for additional operations before it is used in the DW for reporting.

Types of systems

The difference between data warehouse and data mart

Types of data marts

Software tools

The typical extract-transform-load (ETL)-based data warehouse uses staging, data integration, and access layers to house its key functions. The staging layer or staging database stores raw data extracted from each of the disparate source data systems. The integration layer integrates the disparate data sets by transforming the data from the staging layer often storing this transformed data in an operational data store (ODS) database. The integrated data are then moved to yet another database, often called the data warehouse database, where the data is arranged into hierarchical groups often called dimensions and into facts and aggregate facts. The combination of facts and dimensions is sometimes called a star schema. The access layer helps users retrieve data.

Fact (disambiguation)

A fact is an idea which is considered to be wholly and absolutely true.

Fact or Facts may also refer to:

Music

Other uses

See also