"Mad" is a song by American pop/R&B singer Ne-Yo. It is the third single from his album Year of the Gentleman and was produced by Stargate and himself.
The video for the song was directed by Diane Martel. It premiered on AOL on November 25, 2008. The video, shot in black and white, presents a narrative wherein Ne-Yo argues with his girlfriend (played by Faune Chambers). After leaving, in an attempt to save a child from a car accident, someone is fatally hit. At the end of the video, it is revealed that the whole time it has been the ghost of Ne-Yo's character singing, and that it was he who was killed. The video to this song is Part 2 to his song Part of the List.
The music video appears to mirror the 1999 American thriller The Sixth Sense from M. Night Shyamalan, in which Bruce Willis plays a psychologist who is shot by an estranged patient of his. Based on how the film was shot and portrayed, Bruce Willis is shown in many situations as if he is there physically, when in actuality he was murdered by his patient, just as how Ne-Yo appears with his girlfriend, seeming to be there physically and that she is ignoring him due to their earlier argument, when he is actually dead.
Mad1 is a non-essential protein in yeast which has a function in the spindle assembly checkpoint (SAC). This checkpoint monitors chromosome attachment to spindle microtubules and prevents cells from starting anaphase until the spindle is built up. The name Mad refers to the observation that mutant cells are mitotic arrest deficient (MAD) during microtubule depolymerization. Mad1 recruits the anaphase inhibitor Mad2 to unattached kinetochores and is essential for Mad2-Cdc20 complex formation in vivo but not in vitro. In vivo, Mad1 acts as a competitive inhibitor of the Mad2-Cdc20 complex. Mad1 is phosphorylated by Mps1 which then leads together with other activities to the formation of the mitotic checkpoint complex (MCC). Thereby it inhibits the activity of the anaphase-promoting complex/cyclosome (APC/C). Homolog’s of Mad1 are conserved in eukaryotes from yeast to mammals.
In the early 90s, yeast genes were identified which mutations resulted in a defect in mitotic arrest in response to microtubule disassembly (mitotic arrest deficient genes - MAD genes). This cells showed during division no mitotic arrest in the presence of microtubule polymerization inhibitors and were therefore not able to delay cell division. The genes identified included the MAD1, MAD2 and MAD3 genes. They are conserved in all eukaryotes and are involved in a pathway that is active in prometaphase to prevent the premature separation of sister chromatids and constitute the so-called spindle assembly checkpoint(SAC). This checkpoint monitors the status of chromosome attachment to the mitotic spindle and inhibits the metaphase to anaphase transition by preventing the activation of the anaphase-promoting complex/cyclosome (APC/C), and thereby the degradation of cell cycle regulators. Mad1 is in this pathway accumulated at unattached kinetochores and acts as a sensor for unattached kinetochores in this machinery.
Mad2 (mitotic arrest deficient 2) is an essential spindle checkpoint protein. The spindle checkpoint system is a regulatory system that restrains progression through the metaphase-to-anaphase transition. The Mad2 gene was first identified in the yeast S. cerevisiae in a screen for genes which when mutated would confer sensitivity to microtubule poisons. The human orthologues of Mad2 (MAD2L1 and MAD2L2) were first cloned in a search for human cDNAs that would rescue the microtubule poison-sensitivity of a yeast strain in which a kinetochore binding protein was missing. The protein was shown to be present at unattached kinetochores and antibody inhibition studies demonstrated it was essential to execute a block in the metaphase-to-anaphase transition in response to the microtubule poison nocodazole. Subsequent cloning of the Xenopus laevis orthologue, facilitated by the sharing of the human sequence, allowed for the characterization of the mitotic checkpoint in egg extracts.
The Rockman line is a series of headphone amplifiers and other equipment, developed and produced by Scholz Research & Development, Inc. (SR&D), a company formed by Boston founder Tom Scholz. SR&D was sold to Dunlop in 1995. Tom Scholz's signature still appears on the units sold by Dunlop.
The first product released by SR&D was not called a Rockman. It was simply called "Tom Scholz's Power Soak". The Power Soak is an attenuator, put inline between an amplifier and its speakers. Once the guitar amplifier has the right sound, the Power Soak can be used to dial in the output level. The first commercial Power Soak was issued at the end of 1980. An updated version of the Power Soak, the model II, was issued at the end of 1981. It has an extra switch made to adapt the Power Soak to solid-state amplifiers. The Power Soak was re-issued in 1992 as a Power Soak model III, lacking the solid-state switch, though the Power Soak III can be used both with solid-state and tube amps. The jacks were also moved to the back of the unit.
Rockman is a Norwegian music-website, one of Norway's largest with 1.7 million readers. The website began with a music-blog in 2008; its first entry was an editorial review was the story about Led Zeppelin.
Rockman has support from artists, music festival/concert organizers, and other people from the music industry nationally and internationally. Sivert Høyem, lead singer in the former rock band Madrugada said: "Rockman is one such website that Norwegian rock strongly needs".
Mega Man 2, known in Japan as Rockman 2: Dr. Wily no Nazo (ロックマン2 Dr.ワイリーの謎, Rokkuman 2 Dr. Wairī no Nazo, lit. "Rockman 2: The Mystery of Dr. Wily"), is a platform game developed and published by Capcom for the Nintendo Entertainment System. It is the second installment in the original Mega Man series. The game was released in Japan in 1988, and in North America and PAL regions the following years. Mega Man 2 continues the titular protagonist's battle against the evil Dr. Wily and his rogue robots. The game features graphical and gameplay changes from the first Mega Man game, many of which have remained throughout the series.
Although sales for Mega Man were unimpressive, Capcom allowed the Rockman team to create a sequel. They worked concurrently on other Capcom projects, using their free time to develop the game. Unused content from the previous title was integrated into Mega Man 2. Takashi Tateishi composed the soundtrack, with Yoshihiro Sakaguchi serving as a sound programmer.