DNA-PKcs

DNA-dependent protein kinase, catalytic subunit, also known as DNA-PKcs, is an enzyme that in humans is encoded by the PRKDC gene. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family.

Function

DNA-PKcs is the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase called DNA-PK. The second component is the autoimmune antigen Ku. On its own, DNA-PKcs is inactive and relies on Ku to direct it to DNA ends and trigger its kinase activity. DNA-PKcs is required for the non-homologous end joining (NHEJ) pathway of DNA repair, which rejoins double-strand breaks. It is also required for V(D)J recombination, a process that utilizes NHEJ to promote immune system diversity. DNA-PKcs knockout mice have severe combined immunodeficiency due to their V(D)J recombination defect.

Many proteins have been identified as substrates for the kinase activity of DNA-PK. Autophosphorylation of DNA-PKcs appears to play a key role in NHEJ and is thought to induce a conformational change that allows end processing enzymes to access the ends of the double-strand break. DNA-PK also cooperates with ATR and ATM to phosphorylate proteins involved in the DNA damage checkpoint.

PKCS

In cryptography, PKCS stands for "Public Key Cryptography Standards". These are a group of public-key cryptography standards devised and published by RSA Security Inc, starting in the early 1990s. The company published the standards to promote the use of the cryptography techniques to which they had patents, such as the RSA algorithm, the Schnorr signature algorithm and several others. Though not industry standards (because the company retained control over them), some of the standards in recent years have begun to move into the "standards-track" processes of relevant standards organizations such as the IETF and the PKIX working-group.

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PKCS 11

In cryptography, PKCS #11 is one of the Public-Key Cryptography Standards, and also refers to the programming interface to create and manipulate cryptographic tokens.

Detail

The PKCS #11 standard defines a platform-independent API to cryptographic tokens, such as hardware security modules (HSM) and smart cards, and names the API itself "Cryptoki" (from "cryptographic token interface" and pronounced as "crypto-key" - but "PKCS #11" is often used to refer to the API as well as the standard that defines it).

The API defines most commonly used cryptographic object types (RSA keys, X.509 Certificates, DES/Triple DES keys, etc.) and all the functions needed to use, create/generate, modify and delete those objects.

Usage

Most commercial certificate authority software uses PKCS #11 to access the CA signing key or to enroll user certificates. Cross-platform software that needs to use smart cards uses PKCS #11, such as Mozilla Firefox and OpenSSL (using an extension). It is also used to access smart cards and HSMs. Software written for Microsoft Windows may use the platform specific MS-CAPI API instead.

DNA²

DNA² (Japanese: D・N・A² ～何処かで失くしたあいつのアイツ～, Hepburn: Dī En Ei Tsū: Dokoka de Nakushita Aitsu no Aitsu) is a science fiction manga series written and illustrated by Masakazu Katsura. It was serialized across Shueisha's Weekly Shōnen Jump magazine between 1993 and 1994, spanning a total of five tankōbon volumes.

DNA² was adapted into a 12-episode anime television series which ran on Nippon Television from October 7, 1994 to December 23, 1994. This was followed by a three-episode anime original video animation (OVA) in 1995. Produced by Madhouse and Studio Deen, the anime series was directed by Jun'ichi Sakata, whereas the character designer and animation director for the series was Kumiko Takahashi. DNA² has been broadcast in Japan by Animax, which has also aired the series across its respective networks worldwide, including its English-language networks in Southeast Asia and South Asia. All 15 episodes were licensed in North America by Central Park Media until their closing in 2009. The five volumes and box set are out-of-print. Discotek Media has since re-licensed the series for a DVD release in 2014.

DNA2.0

DNA2.0 provides products and services for life science and Synthetic biology research. DNA2.0 also provides free access to research tools such as Gene Designer, DNA Atlas and a gRNA designer.

The company

DNA2.0 was founded in 2003, in Menlo Park, California. The company is privately held and continues to have all research, development and production in Menlo Park, California. It began and continues as a gene synthesis and protein engineering provider to academia, government and the pharmaceutical, chemical, agricultural and biotechnology industries. Gene Synthesis rapidly replaced molecular cloning for many academic and corporate labs, as "foundries for the biotechnology age" allowing made-to-order genes for biological research. DNA2.0 was featured on the PBS show Nova ScienceNow to show how genes are created synthetically in a lab. In 2008, the company supplied some of the DNA stretches used to create a synthetic bacterial genome.Dan Rather Reports included DNA2.0 in their episode on Synthetic Biology and how it is solving "some of the most important problems facing the world." In 2009, The Scientist named the codon design algorithms (now tradmarked as GeneGPS) developed by DNA2.0 as one of the Top 10 Innovations of the year for Life Sciences. DNA2.0 developed the Electra Vector System, a universal cloning system that utilizes the type IIS restriction enzyme SapI and T4 DNA ligase in a single-tube reaction. DNA2.0 has made some molecular components, such as synthetic fluorescent proteins, available in open-access collections of DNA parts (BioBricks Foundation). DNA2.0 is a founding member of the International Gene Synthesis Consortium (IGSC) to promote biosecurity in the gene-synthesis industry. There are over 1,100 published scientific articles using DNA2.0 products and/or services, of which 43 include company employees as an author(s).

DNA (magazine)

DNA is an Australian monthly magazine targeted at the gay male audience. The magazine features stories, celebrity profiles, pop culture reviews, fashion tips/reviews, grooming tips and photography. The magazine is available at most newsagencies in Australia, as well as larger book stores. Launched in Australia in 2000, the magazine is now available in many countries, including Canada, the United States, New Zealand, United Kingdom and several other countries in Europe.

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