Please tell us which country and city you'd like to see the weather in.
Dige
Dige (simplified Chinese: 底阁镇; traditional Chinese: 底閣鎮; pinyin: Dǐgé Zhèn) is a town in the south of Shandong province, People's Republic of China, bordering Jiangsu province to the south, and located in the southeast corner of Zaozhuang City. It is under the administration of Yicheng District and is 45 kilometres (28 mi) east-southeast of downtown Zaozhuang. In March 2001, the former Ganlugou Township (甘露沟乡) was merged into the town's administrative area.
References
Difference gel electrophoresis
Difference gel electrophoresis (DIGE) is a form of gel electrophoresis where up to three different protein samples can be labeled with size-matched, charge-matched spectrally resolvable fluorescent dyes (for example Cy3, Cy5, Cy2) prior to two-dimensional electrophoresis. Then, the three samples are mixed and loaded onto IEF for first dimension and the strip is transferred to a SDS PAGE. After the gel electrophoresis, the gel is scanned with the excitation wavelength of each dye one after the other, so we are able to see each sample separately (if we scan the gel at the excitation wavelength of the Cy3 dye, we will see in the gel only the sample that was labeled with that dye). This technique is used to see changes in protein abundance (for example, between a sample of a healthy person and a sample of a person with disease), post-translational modifications, truncations and any modification that might change the size or isoelectric point of proteins. The binary shifts might be left to right (change in isoelectric point), vertical (change in size) or diagonal (change in both size and isoelectric point). Reciprocal Labeling is done to make sure the changes seen are not due to dye-dependent interactions.
Podcasts:
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by Djay
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by Djay
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by Djay
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by Taj
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by Tajai
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by Taj
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by Tajai
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by Djou'e
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by Teejay
Hustle And Flow (it Ain't Over)
by: Djay[singer hums and ad libs for 22 seconds]
[Djay]
Look this is my life, and it's a battle within
I gotta survive, even if I'm sinnin to win
And if I show no remorse I reap the devil's reward
He said he'd, give me riches but I'm lookin for more
When I was young, witnessed my dad, standin for right
Black pride in him even though he passin for white
Took years from my life, now I'm missin the man
Moms on some other shit and now I'm missin the plan
And so I'm... stuck in this fuck-a-marole
All the lessons to a young teen baby was cold
Then my pimpin-ass Uncle put me up on the game
It really ain't no love it's 'bout this, paper mayne
Put me in a position, got me out on a mission
Collectin from the hoes turned on to my pimpin
A nigga from Memphis dealin with life as a struggle
This is the gift I was given so I just live by my hustle
[Chorus x2: Djay (singer, ad libbing at times)]
(Keep hustlin) It ain't over for me, no it ain't over for me
(Keep, flowin) I'ma step my game up and get what's comin to me
[Djay]
Now I'm just gettin by, and it's FUCKIN with me
See others doin big thangs they was nothin to me
I'm seein what I used to be, and it's lookin legit
Got my hands on some music, started FUCKIN with it
Put it down hard, then I started bumpin to this
I let some others get a dose and they was NUTTIN to this
Let me check my connections cause we go back in the day
Say they was lookin for 'dro, heard I was hustlin hay
Got me connin my niggaz, spittin game to my hoes
This my opportunity I feel like anything goes
I was never 'sposed to see the new millenium mayne
And I refuse to let my dad die in vain
So I give it my all, you feel my struggle and pain
If you ain't feelin what I'm spittin trick you late to the game
But the closest ones to me know I'm holdin it down
You gon' feel me one day, we gon' be heard in the town