Fragment antigen-binding

The fragment antigen-binding (Fab) fragment is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. The variable domain contains the paratope (the antigen-binding site), comprising a set of complementarity determining regions, at the amino terminal end of the monomer. Each arm of the Y thus binds an epitope on the antigen.

In an experimental setting, Fc and Fab fragments can be generated in the laboratory. The enzyme papain can be used to cleave an immunoglobulin monomer into two Fab fragments and an Fc fragment. The enzyme pepsin cleaves below hinge region, so a F(ab')₂ fragment and a pFc' fragment is formed. Recently another enzyme for generation of F(ab')₂ has been commercially available. The enzyme IdeS (Immunoglobulin degrading enzyme from Streptococcus pyogenes, trade name FabRICATOR) cleaves IgG in a sequence specific manner at neutral pH. The F(ab')₂ fragment can be split into two Fab' fragments by mild reduction.

Fab (website)

Fab is an e-commerce company founded in 2010. Once estimated at a worth of over 1 billion dollars, in November 2014, the company was rumored to be in talks to sell for 15 million. In March 2015, PCH International acquired Fab for an undisclosed sum.

It grew from 175,000 members in June 2011, to over 10 million as of December 2012. As of April 30, 2013, Fab has disclosed that it receives 6 million unique visitors per month and have sold over 7 million products since launch. Fab is headquartered in Manhattan and operates a warehouse in Keasbey, New Jersey. The company has office locations in NYC, Pune (India), and Berlin.

History

Fab was founded in February 2010 by Jason Goldberg (entrepreneur) (formerly of XING AG and Jobster) and chief designer Bradford Shellhammer. The site was originally created as a social network for gay men and their friends before pivoting on June 9, 2011 into its model of daily design inspirations and sales. In November 2011, five months after re-launching, Fab reached 1 million members. The company reached this number faster than Facebook, Twitter and Groupon. In September 2012, Fab removed the membership requirements to browse the site. In December 2012, Fab passed 10 million members, up from 7.5 million members in September. Fab also announced it had sold more than 4.3 million products since its launch, averaging 5.4 products sold per minute. In July 2013, fab eliminated 100 positions at its Berlin office as a profit-building measure. Fab laid off 101 more staff, primarily at its New York office in October 2013, further reducing their workforce by 19 percent.

Uniregistry

Uniregistry is a Cayman Islands-based domain name registry that administers the generic top-level domains .audio, .auto, .blackfriday, .car, .cars, .christmas, .click, .diet, .flowers, .game, .gift, .guitars, .help, .hiphop, .hiv, .hosting, .juegos, .link, .lol, .mom, .photo, .pics, .property, .sexy, and .tattoo. In February 2012, the related company Uniregistrar Corporation became an ICANN-accredited registrar and launched under the licensed Uniregistry brand name in 2014.

History

Uniregistry Corporation was officially founded in 2012 by Frank Schilling, one of the largest private domain name portfolio owners in the world, and registered in the Cayman Islands. However, the domain Uniregistry.com was registered six years earlier and the company filed an intent to use the name in the Cayman Islands in 2010. Trademark applications for the "Uniregistry" mark and its stylized "U" logo were filed in 2012. That year, Schilling invested $60 million and applied for 54 new top-level domains. Uniregistrar Corporation became an ICANN-accredited registrar in February 2013. In January 2014, Uniregistry Inc. became a subsidiary in Newport Beach, California to house a West Coast service and support team. The registrar began operating under the licensed Uniregistry brand name in 2014. Uniregistry's registry infrastructure was designed by Internet Systems Consortium (ISC) and Uniregistry subsequently purchased its infrastructure in 2013.

Gift (Kanjani Eight EP)

"GIFT" is a triple-single released by the Japanese boyband Kanjani Eight—stylized Kanjani∞. The three singles are the band's 11th, 12th, and 13th singles. The songs were released during the Christmas period. Each single release, except "Green", contains two songs; Green has three songs. Each single has cover art reflecting the color theme of each single. GIFT was advertised as a gift from the group members to their fans. The three singles were sold at a special price of ¥888 and were a limited-press release.

The "GIFT" singles have seven Christmas-themed songs; some were written or composed by members of the group. Shota Yasuda designed the label for the single; Kanjani8 collectively photographed the backstage activities and provided creative ideas for the jacket design. Like the previous singles since "It's My Soul", member Tadayoshi Okura dresses as Santa Claus as the singles' joke for this release. He appears in the outfit on "Gift ~Green~", completing the group picture that spans the backs of all three singles.

Gift (Taproot album)

Gift is the first major label album by the rock group Taproot. It was released on June 27, 2000. "I" and "Again & Again" were minor Mainstream Rock singles. The album has sold at least 250,000 copies.

Music and lyrics

Described as a nu metal album, the album's lyrical topics are primarily about topics such as depression and disliking life. Taproot's vocalist Stephen Richards' vocals on the album Gift have been compared to vocalists such as Chino Moreno, Trent Reznor, Jonathan Davis and Mike Patton. The album consists of vocal styles such as singing, rapping, growling and screaming. The album features characteristics that have been compared to Tool, Incubus, Deftones, Glassjaw, The Cure, Depeche Mode, Outkast, P.O.D., Faith No More, Slipknot and Papa Roach.

Track listing

Notes