DERB

Dual expression recombinase based (DERB) single vector system is a method of efficient cloning and subcloning of plasmid vectors for high throughput screening (HTS) and verification of protein-protein interactions inside living cells. DERB was developed by Lu JP et al.

The DERB process

Plasmid Vectors are deliberately constructed circular double-strand DNA loops capable of self-amplification and protein production. They are widely used in laboratories and the bio-medical and pharmaceutical industries to produce of DNA or protein in quantity. The DERB vector system consists of a series of vectors, each of which produces two or more proteins which are labeled or tagged for screening and verification of molecular interactions such as protein–protein interactions.

The following characteristics of the vectors ensure highly efficient cloning or subcloning of the protein of interest ORFs into the DERB vectors for high-throughput screening (HTS) and verification of protein–protein interactions after single introducing of the vectors into eukaryotic or prokaryotic cells:

Thunderpuss

Thunderpuss was the (mainly dance) remix/production team of Los Angeles based music producers Barry Harris and Chris Cox. Harris had previously worked as part of several music groups including Kon Kan, Top Kat, Killer Bunnies and Outta Control and had also released several solo singles himself. Cox, a musician and DJ, had also worked for years as a DJ and producer, producing a megamix of Paula Abdul songs, among other things, and started his own record label, Interhit Records, with Jeff Johnson.

History

Harris and Cox originally met in New York City in 1990 while Harris was recording Syntonic, Kon Kan's second album. They became friends and remained in contact for years, with Harris eventually joining Cox at Interhit Records in Los Angeles in January 1998. They didn't start working together as a production team until September 1997. Their first project as Thunderpuss was a cover of the song "I Just Wanna Be Your Everything", with Thea Austin, formerly of Snap! performing vocals. They also produced Engelbert Humperdinck's 1998 album The Dance Album, but did not use the name Thunderpuss. They also produced original songs for various artists, including Abigail ("Let the Joy Rise" and "If It Don't Fit"), Amber ("Taste the Tears") and Soluna. During this time, they also released remix singles under the name 'Thunderpuss 2000', most notably covers of David Bowie's "Heroes" and Blue Öyster Cult's "Godzilla".

Thunderpuss (album)

Thunderpuss is a compilation album mixed by DJs and producers Barry Harris and Chris Cox, better known under the moniker Thunderpuss. Released in 2002, it comprises their remixes of other artists' work as well as their own music.

Track listing