Dual expression recombinase based (DERB) single vector system is a method of efficient cloning and subcloning of plasmid vectors for high throughput screening (HTS) and verification of protein-protein interactions inside living cells. DERB was developed by Lu JP et al.
Plasmid Vectors are deliberately constructed circular double-strand DNA loops capable of self-amplification and protein production. They are widely used in laboratories and the bio-medical and pharmaceutical industries to produce of DNA or protein in quantity. The DERB vector system consists of a series of vectors, each of which produces two or more proteins which are labeled or tagged for screening and verification of molecular interactions such as protein–protein interactions.
The following characteristics of the vectors ensure highly efficient cloning or subcloning of the protein of interest ORFs into the DERB vectors for high-throughput screening (HTS) and verification of protein–protein interactions after single introducing of the vectors into eukaryotic or prokaryotic cells:
mama, lick on me now
no care
put me on the bad side
oh, so bad
i wanna make you love
wanna, wanna watch your eyes
dip them in a honey glass
mirror mine
roll them high
wanna make them
drip away
trip away
trip away
trip away
trip away
trip away
way out
way out
oh, mama lick on me
i'm as tasty as a red plumb
baby thumb
wanna make you love
wanna be wild animals
lay down in a shady den
see the spring
color in
watch the stoney winter
drip away
trip away
trip away
trip away
trip away
trip away
way out
way out
take my eye
do not wonder why
i've come to the crux
i've looked it up and down
seen the trespassers trample themselves
as the miser makes love with his wealth
convenience his love!
tripped away the summer days
some are gone
some are o.k.
summers gone
some are makin love
i love to watch you
love to watch you
drip away
trip away
trip away
trip away
trip away
trip away
way out