Dioctadecyldimethylammonium bromide (DODAB) is a doublechained quaternary ammonium surfactant that forms unilamellar vesicles (ULVs) in water. Among various preparation methods, the ‘‘hot-water” method offers a simple procedure to prepare DODAB cationic vesicles by simply dissolving the DODAB in hot water above 50 °C, i.e., chain melting (main) transition, Tm. In general, the DSC thermograms of the unsonicated DODAB dispersions are dominated by two endotherms; the pre- (35–36 °C) and main transition (42.7–45 °C) peaks. Moreover, in literature reported the presence of a third endotherm (post transition) at 52.2 °C. The main transition (Tm) is ascribed to gel to liquid-crystalline phase transition in which the alkyl chains transform from solidlike to liquid-like state.
The 10 mM DODAB is a critical concentration, below which the dispersions consist of large polydispersed unilamellar vesicles (ULVs) that exhibit a local (chain melting) transition at 43 °C, beyond which a structural transition occurs: ULVs --> MLVs (multilamellar vesicles) as indicated by the sudden increase in the dynamic moduli. However, above 10 mM DODAB, the dispersions are mostly formed by ULVs in coexistence with lamellar fragments resulting in a network that shows a rheogram similar to that of hexagonal liquid-crystalline phase.
[Chorus:]
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[Chorus:]
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I'll be getting to the money [?] 10 summaz.
My money loan like 10 [?]
That nigger full of shit, somebody call the plumber
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[Chorus:]
10 summaz, 10 sumaz,
I'll be getting to the money [?] 10 summaz.
My money loan like 10 [?]
That nigger full of shit, somebody call the plumber
A nigger hot like 10 summaz,
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I'll be getting to the money that's ten [?]
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[Chorus:]
10 summaz, 10 sumaz,
I'll be getting to the money [?] 10 summaz.
My money loan like 10 [?]
That nigger full of shit, somebody call the plumber!
A nigger hot like 10 summaz,
10 chains [?] ain't walking on the [?]
My money loan like 10 [?]