DERB
Dual expression recombinase based (DERB) single vector system is a method of efficient cloning and subcloning of plasmid vectors for high throughput screening (HTS) and verification of protein-protein interactions inside living cells. DERB was developed by Lu JP et al.
The DERB process
Plasmid Vectors are deliberately constructed circular double-strand DNA loops capable of self-amplification and protein production. They are widely used in laboratories and the bio-medical and pharmaceutical industries to produce of DNA or protein in quantity. The DERB vector system consists of a series of vectors, each of which produces two or more proteins which are labeled or tagged for screening and verification of molecular interactions such as protein–protein interactions.
The following characteristics of the vectors ensure highly efficient cloning or subcloning of the protein of interest ORFs into the DERB vectors for high-throughput screening (HTS) and verification of protein–protein interactions after single introducing of the vectors into eukaryotic or prokaryotic cells:
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Therapy
by: Therapy?I'm trying to walk up the stairs
My hands are snatchin' at the slivers of light
I'm stickin' to the steps
Each one a release
From the place below
I'm on a mission
On the hunt for clean
Clear vapour skies
'Cos I'm choking on my own
I need some air
The dark skies behind me
Begging you to scrape off, your disco paint
It's open to the night
And I'm sick as a hospital
And empty factories
You look so tiny, so very unimportant
I'm nearly there, and everything feels fine
Don't wanna look behind me
Don't wanna look beneath me
Every movement
Every vibration
Every movement
Every vibration
High above piss city
Watch the pigs-ear people
All dead in their drows
Some suffer in silence
Someone sparks
Some gorge sucking on silicone
I've got the urge to jump
Watch life whizz by
Fast foward flash
But hold on boy
I feel... alive
Don't wanna look behind me
Don't wanna look beneath me
Every movement
Every vibration
Every movement
Every vibration
Don't wanna look behind me
Don't wanna look beneath me
Every movement
Every vibration
Every movement
Every vibration
Don't wanna look behind me
Don't wanna look beneath me
Every movement
Every vibration
Every movement
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