Morgan

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Ah! My Goddess: The Movie

Ah! My Goddess: The Movie (劇場版「ああっ女神さまっ」, Gekijōban "Aa! Megami-sama!") is a 2000 Japanese animated film directed by Hiroaki Gōda, written by Michiko Yokote and Yoshihiko Tomizawa, animated by Anime International Company and co-produced by Kodansha, Dentsu, Sega Enterprises, Pony Canyon, Nippon Shupan Hanbai, AIC, MOVIC and Shochiku, based on the manga of the same name by Kōsuke Fujishima. The film's theatrical release took place on October 21, 2000. It was also distributed by Shochiku in Japan. The film was licensed by Geneon Entertainment in the North America, MVM Films in the United Kingdom, and Madman Entertainment in Australia and New Zealand.

Plot

The story takes place during the third spring since Belldandy's arrival. Celestin, a former mentor of Belldandy, is released by Morgan Le Fay, and erases all memories of Keiichi from Belldandy to use her as a virus to hack the Yggdrasil computer in the heavens.

Morgan le Fay heads to the Lunar Prison on the moon and releases the seal. She is able to make contact with a small mask that reveals that they must unite for their common goal.

Morgan (orca)

Morgan is a female orca who was rescued in the Wadden Sea, off the northwestern coast of the Netherlands in June 2010. She was found in terrible condition, severely underweight and malnourished. She lived several months in the Dolfinarium Harderwijk in the Netherlands. After it became clear that she could not be released to the wild because her pod wasn't able to be located, the Dolfinarium worked hard to find another facility for her because their basin was too small. However, activists set up a battle and went to court. The whole business extended Morgan's time at the Dolfinarium and she spent a year longer in her small basin. Eventually the court decided that she was to be moved and she was transported to the Loro Parque in Spain.

History

The orca, now named Morgan, was administered medical assistance and provided with food. The reason for the orca's capture was emaciation (weighing approximately 430 kg) and likely also dehydration. Her body length was 3.5 m.

Public Controversy

Clip (film)

Clip (Serbian: Klip, Клип) is a 2012 Serbian drama film directed by Maja Miloš and tells the story about young teenager girl Jasna who is obsessed with sex. The film became controversial for its depiction of some explicit scenes, supposedly performed by then fourteen years old actress Isidora Simjonović. However, the authenticity of those scenes is still debatable.

The director said in her interviews that Simjonović had been exposed to some degree of explicitness, however Miloš didn't tell how much of it nor to what kind of sexual acts. Also, Miloš said that prosthetic, dildos, special visual effects, body doubles had also been used, as well as the film had had long post production period but actually, she said nothing about unsimulated sex. However, due to the both film's hyper-realism and the use of minors as the main actors, film was banned in Russia as being a child pornography.

The film was released in Serbia on April 12, 2012 at Belgrade's Sava Center. It won KNF Award and Tiger Award at the 2012 Rotterdam International Film Festival.

CLIP2

CAP-Gly domain-containing linker protein 2 is a protein that in humans is encoded by the CLIP2 gene.

The protein encoded by this gene belongs to the family of cytoplasmic linker proteins, which have been proposed to mediate the interaction between specific membranous organelles and microtubules. This protein was found to associate with both microtubules and an organelle called the dendritic lamellar body. This gene is hemizygously deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants.

References

Further reading

CLIP

CLIP (cross-linking immunoprecipitation) is a method used in molecular biology that combines UV cross-linking with immunoprecipitation in order to analyse protein interactions with RNA. CLIP-based techniques can be used to map RNA binding sites for a protein of interest on a genome-wide scale, thereby increasing the understanding of post-transcriptional regulatory networks.

Workflow

CLIP begins with the in-vivo cross-linking of RNA-protein complexes using ultraviolet light (UV). Upon UV exposure, covalent bonds are formed between proteins and nucleic acids that are in close proximity. The cross-linked cells are then lysed, and the protein of interest is isolated via immunoprecipitation. In order to allow for sequence specific priming of reverse transcription, RNA adapters are ligated to the 3' ends, while radiolabeled phosphates are transferred to the 5' ends of the RNA fragments. The RNA-protein complexes are then separated from free RNA using gel electrophoresis and membrane transfer. Proteinase K digestion is then performed in order to remove protein from the RNA-protein complexes. This step leaves a peptide at the cross-link site, allowing for the identification of the cross-linked nucleotide. After ligating RNA linkers to the RNA 5' ends, cDNA is synthesized via RT-PCR. High-throughput sequencing is then used to generate reads containing distinct barcodes that identify the last cDNA nucleotide. Interaction sites can be identified by mapping the reads back to the transcriptome.