Tisamenis was of Athenian descent. Her ancestry can be traceable to the Athenian noble woman Elpinice (a half sister of statesman Cimon and daughter of politician Miltiades the Younger). She had an ancestor four generations removed from her called Polycharmus. Polycharmus from 9/8 BC-22/23, could have served as an Archon of Athens. Her family bears the Roman family name, Claudius. There is a possibility that a paternal ancestor of hers, received Roman citizenship, from an unknown member of the Claudius gens.