Human Herpes Virus (HHV) Infected Cell Polypeptide 0 (ICP0) is a protein, encoded by the DNA of herpes viruses. It is produced by herpes viruses during the earliest stage of infection, when the virus has recently entered the host cell; this stage is known as the immediate-early or α ("alpha") phase of viral gene expression. During these early stages of infection, ICP0 protein is synthesized and transported to the nucleus of the infected host cell. Here, ICP0 promotes transcription from viral genes, disrupts structures in the nucleus known as nuclear dots or promyelocytic leukemia (PML) nuclear bodies, and alters the expression of host and viral genes in combination with a neuron specific protein. At later stages of cellular infection, ICP0 relocates to the cell cytoplasm to be incorporated into new virion particles.
ICP0 was identified as an immediate-early polypeptide product of Herpes simplex virus-1 (HSV-1) infection in 1976. The gene, in HSV-1, from which ICP0 is produced is known as HSV-1 α0 ("alpha zero"), Immediate Early (IE) gene 1, or simply as the HSV-1 ICP0 gene. The HSV-1 ICP0 gene was characterized and sequenced in 1986. This sequence predicted a 775 amino acid sequence with a molecular weight of 78.5 KDa. At the time of gene isolation, ICP0 was known as IE110 as gel electrophoresis experiments performed prior to obtaining the gene sequence indicated the ICP0 protein weighed 110 kDa. Post-translational modifications, such as phosphorylation or sumoylation, were presumed to account for the actual protein size appearing 30 kDa larger than that of the predicted amino acid sequence.
Sharon G. Flake (born December 24, 1955) is an American writer of young adult literature. She has lived in Pittsburgh, Pennsylvania, with her daughter for many years. She is a graduate of the University of Pittsburgh with a BA in English.
Her debut novel The Skin I'm In (1998) follows a young African American girl who has issues with people teasing her about the color of her skin and the way she dresses. She tries her best to fit in but it doesn't go too well. She learns that trying to fit in is not the best way to make people like you. Her works have won numerous awards. The Skin I'm In won the John Steptoe Award for New Talent in 1999 for new authors and garnered positive feedback from Booklist and School Library Journal. She has been a runner-up for two Coretta Scott King Awards.
Flake was born in Philadelphia. She is the second youngest child, with three brothers and two sisters, and grew up in an inner-city neighborhood. Her father worked for Philadelphia Gas Co., while her mother did days work and raised her children. Through their guidance, Flake and her siblings were encouraged to be themselves, learning about culture through music, TV, politics, and books. As a teenager, she attended Simon Gratz High School, where she was a member of the tennis team.She wrote many books.
"Bang" is a song and single by rapper/dancer Rye Rye featuring M.I.A.. It was recorded in 2008 and appears on the deluxe version of her 2012 album Go! Pop! Bang!. It was released in 2009 on N.E.E.T. Recordings and Interscope Records. The single was released first, followed by an EP of remixes, Bang – The Remixes, which includes remixes by Buraka Som Sistema and DJ Sega. The song appears in the films Fast & Furious and Step Up 3D and in the episode "The Sorkin Notes" of the TV-series Entourage.
The video features Rye Rye, M.I.A. and several Baltimore club dancers including Whyte Boi dancing in a dark, messy underground club setting. The video was directed by M.I.A. in Baltimore.
Bang! (also known as The Gun Game! amongst other variants) is a drama game, in which players stand in a circle and shoot each other with imaginary guns.
The objectives of the game include enhancing concentration skills and reaction time as well as helping groups of people remember each other's names.
The workshop leader/director or other nominated person does not join the circle. Each remaining player holds an imaginary gun in each hand, pointed at the adjacent player.
The leader will call out a player's name. If he/she calls "John" then John must duck to avoid being shot. The players on either side of John must shout "bang!" One of the following then occurs:
If someone accidentally ducks or shoots when they were not supposed to be doing so then they are also out.