Mitotic checkpoint serine/threonine-protein kinase BUB1 also known as BUB1 (budding uninhibited by benzimidazoles 1) is an enzyme that in humans is encoded by the BUB1 gene.
Bub1 is a serine/threonine protein kinase first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of Bub1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence.
Bub1p comprises a conserved N-terminal region, a central non-conserved region and a C-terminal serine/threonine kinase domain. The N-terminal region mediates binding of Hs-BUB1 to the mitotic kinetochore protein blinkin (a protein also commonly referred to as AF15q14). The latter interaction is essential for kinetochore localization of Bub1 and its function in cell cycle arrest induced by spindle assembly checkpoint (SAC) activation. The crystal structure of human Bub1 revealed the presence of a N-terminal tetratricopeptide repeat (TPR) domain and a C-terminal kinase domain (residues 784–1085), adopting a canonical kinase fold with two lobes. The ATP binding and the catalytic sites are located at the interface of the two lobes. The N-terminal extension contains three β-strands and an α-helix, wrapping around the N lobe of the kinase domain.
This is the way I live.
Lil' Boy still pushin' big wheels
I stack my money, lay low, and chill.
Don't need to work hard that's the way I feel, I feel, I
This is the way I live.
Lil' Boy still pushin' big wheels
I stack my money, lay low, and chill.
Don't need to work hard that's the way I feel, I feel, I
This is the way I live.
Six months later now we're big dog status.
Name elevated but I still act average.
Rockin' shows in Dallas, ball like I play for the Mavericks.
West Bank rapper and I'm one of the baddest.
French Rivera spot and I'm getting in shape man.
I think I'm Super M-a-n without a cape man.
Ridin' all day hustlin'; all for the cake man.
I'm a Benjamin stack one inch taller than Great Danes.
First they made cheap Ray-Ban like a felon.
I'm growin' up now spittin' hotter than Mohellans
I'm a steal every girl ever gon' to fly.
I'm a young little dude, stay runnin' from cops.
Emerald Row is the place where I got my props.
People love me in da hood they don't want me to stop.
So uhh, that's the only way I know how to rock.
And I'm a stay rappers like won't stop ‘til I drop.
This is the way I live.
Lil' Boy still pushin' big wheels
I stack my money, lay low, and chill.
Don't need to work hard that's the way I feel, I feel, I
This is the way I live.
Lil' Boy still pushin' big wheels
I stack my money, lay low, and chill.
Don't need to work hard that's the way I feel, I feel, I
This is the way I live.
I I's Baby Boy and I done it again.
I'm ridin fly with D-Wizzle in ‘cedes Benz
Twenty two wanna ride with the ladies then
Red Bull, Grey Goose, now the ladies bent
Now we go and do a thing under a shady tent
And they don't question what I say cause I'm a ladies pimp.
See this little one right here this fellow got stripes.
And I is represent, my rail room for life.
Money in my pocket but I still act trife
While I in da club they say the parlor a'int right.
Look I'm-doing great that's the way I feel.
Hustlin' hard all day that's the way I live.
This is the way I live.
Lil' Boy still pushin' big wheels
I stack my money, lay low, and chill.
Don't need to work hard that's the way I feel, I feel, I
This is the way I live.
Lil' Boy still pushin' big wheels
I stack my money, lay low, and chill.
Don't need to work hard that's the way I feel, I feel, I
This is the way I live.
This the two-seat rider with the ketchup inside.
Red pepper paint must believe my ish ride.
They pause like stop signs when they rollin' up.
Cause' when the doors open up, they open UP.
I'm Lamboin' it I was stashed, off stage I'm throwin' it.
Cause they say I'm like Katrina with money, I blow the shhhh.
Real been gravy, three years out of da eighties.
Solos, shabo's valley animals played it.
Commodores mirages, not the Nike hirages.
It cost 250 on up to put your foot inside this.
Y'all was wearing rocks that was my time to shine.
Don't give me viesels y'all thinkin' this McDonalds size.
I been fly I I I I I I I I from the crib to the ri I I I I I de
Fifty-Four inch screens 26 inches from the ceiling dude.
The plasma look like a projector up in da livin' room homie!
This is the way I live.
Lil' Boy still pushin' big wheels
I stack my money, lay low, and chill.
Don't need to work hard that's the way I feel, I feel, I
This is the way I live.
Lil' Boy still pushin' big wheels
I stack my money, lay low, and chill.
Don't need to work hard that's the way I feel, I feel, I