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Anth

Anth is a 1994 Indian action film directed by Sanjay Khanna and produced by Ashok Honda and starred Sunil Shetty and Somy Ali in pivotal roles. Paresh Rawal, Deepak Shirke, Mohan Joshi, Makrand Deshpande, Alok Nath and Vijayendra Ghatge also featured in the film.

Plot

Kali (Makrand Deshpande) is a local ruffian who harasses students in his college. The principal Satyaprakash (Alok Nath) is helpless to stop him because Kali is the son of Dabala (played by Paresh Rawal) who is one of the biggest gangsters in town. The principal has two sons and a daughter - Vikas (Vijayendra Ghatge), Vijay (Sunil Shetty) and Pooja. Vijay is in love with Priya (Somy Ali) a colleague of his in his engineering company.

Kali rapes Pooja, and then the corrupt inspector Shirke (Deepak Shirke) puts the blame on Pooja's fiancee, and has him killed while in custody. This enrages Vijay and he embarks upon a bloody and violent fight to cleanse the city of ruffians like Kali and Dabala and Shirke. (Mohan Joshi) shows up in a cameo as an honest ACP.

ANTH domain

The ANTH domain is a membrane binding domain that shows weak specificity for PtdIns(4,5)P2. It was found in AP180 (a.k.a. CALM) endocytotic accessory protein that has been implicated in the formation of clathrin-coated pits. The domain is involved in phosphatidylinositol 4,5-bisphosphate binding and is a universal adaptor for nucleation of clathrin coats.

Its structure is a solenoid of 9 helices. The PtdIns(4,5)P2 binding residues are spread over several helices at the tip of the structure. The PtdIns(4,5)P2 binding sequence is Kx9Kx(K/R)(H/Y).

An ANTH domain is also found in HIP1 and HIP1R, and the PtdIns(4,5)P2 binding sequence is conserved. More information is found on endocytosis.org.

Human proteins containing this domain

HIP1; HIP1R; PICALM; SNAP91;

References

Further reading

  • Ford MG, Pearse BM, Higgins MK; et al. (February 2001). "Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes". Science 291 (5506): 1051–5. doi:10.1126/science.291.5506.1051. PMID 11161218. 
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