Alone (Why Must I Be Alone)

"Alone (Why Must I Be Alone)" is a popular song written by Morty Craft. Craft owned a record label, and produced the recording by the Shepherd Sisters on that label. The lyrics were written by Craft's wife, Selma.

While it was the only hit for The Shepherd Sisters (as The Sheps) in the United States (reaching #18 on the Billboard charts on November 11, 1957), in the United Kingdom it was one of a number of hits for Petula Clark (reaching #8 on the UK charts) before she became famous internationally. The Shepherd Sisters' version also charted in the UK, reaching #14, and another version, by The Southlanders, reached #17 on the UK chart.

A remake of the song by The Four Seasons charted in 1964, reaching its peak Billboard Hot 100 position at #28, on July 18. "Alone (Why Must I Be Alone" also went to #8 on the Canadian singles chart. It was the act's last hit single on Vee Jay Records, as The Four Seasons left the label at the beginning of 1964 in a royalty dispute. The Four Seasons recording of the song features a refrain that is whistled.

Alone (Solitude Aeturnus album)

Alone is the sixth album by the US doom metal band Solitude Aeturnus. It was released in 2006. The album cover is done by Travis Smith.

Track listing

Credits

Line-up

References

Love Amongst Ruin (album)

Love Amongst Ruin is the self-titled debut studio album by Love Amongst Ruin. It was released on September 13, 2010.

Background

Writing and recording

After departingPlacebo in October 2007, Steve Hewitt enlisted Lamb bassist Jon Thorne and his brother Nick Hewitt to begin writing and demoing new music at his home studio. Hewitt explained that he decided to write with Jon Thorne because he "wanted to play rock drums against somebody playing upright bass. And that’s what we did and the first thing we ever wrote was "Running"".Julian Cope collaborator Donald Ross Skinner was brought in to oversee and co-produce the recording sessions and the collective relocated to Moles Studio in Bath for three recording sessions with producer Paul Corkett over the summer of 2008. The sessions yielded ten songs, on which Steve performed drums and lead vocals. Mixing began in September and continued for six months before the album was mastered by Brian Gardner in April 2009.

"Bring Me Down (You Don't)" was to be included on the album, but legal trouble with publishers of the band Can resulted in the track being replaced with "Come On Say It". An acoustic version of the song was later released for free via SoundCloud in November 2011. "Come On Say It" featured then-band members Steve Hove, Laurie Ross and Keith York and was mixed ten weeks before the album's release. Other songs which were recorded, but didn't make the cut for the album, were cover versions of "Got To Give It Up" (Thin Lizzy) and "Rise" (Public Image Ltd), the latter being released for free via SoundCloud in July 2012.

Argonaute

The Argonaute protein family plays a central role in RNA silencing processes, as essential catalytic components of the RNA-induced silencing complex (RISC). RISC complex is responsible for the gene silencing phenomenon known as RNA interference (RNAi). Argonaute proteins bind different classes of small non-coding RNAs, including microRNAs (miRNAs), small interfering RNAs (siRNAs) and Piwi-interacting RNAs (piRNAs). Small RNAs guide Argonaute proteins to their specific targets through sequence complementarity (base pairing), which then leads to mRNA cleavage or translation inhibition.

Discovery

The RNA interference (RNAi) was first reported in 1995 by Guo and Kemphues, and similar pathways collectively referred to as RNA silencing were discovered in plants and fungi. The beginning of people’s understanding of the mechanism of RNA silencing began only in 1998 with the experiments of Fire and colleagues demonstrating that double-stranded RNA triggered RNAi. RNA silencing pathways process long RNAs into small RNAs that direct the repression of transcription or translation of nucleic acid targets with sequence corresponding to the small RNAs. These single-stranded RNAs, referred to as guide strands, are incorporated into RNA silencing effectors complexes such as the RNA-induced silencing complex (RISC). These RNA silencing effector complexes contain Argonaute family proteins.