Gothika

Gothika is a 2003 supernatural horror film directed by Mathieu Kassovitz and written by Sebastian Gutierrez. Halle Berry plays a psychiatrist in a women's mental hospital who wakes up one day to find herself on the other side of the bars, accused of having murdered her husband.

The film was first released on November 21, 2003 in the United States. At the time of its release, Gothika was the most successful film from Dark Castle Entertainment with $141.6 million.

Plot

Psychiatrist Dr. Miranda Grey (Halle Berry) works at a mental hospital and has a car accident after trying to avoid a girl (Kathleen Mackey) on a road during a stormy night while driving back home. She rushes to try to help the girl. The girl turns out to be a ghost and possesses Miranda's body by burning her after she extends her hand to the girl. Miranda loses consciousness. Miranda next wakes up in the very hospital she works for, but as a patient treated by her co-worker, Dr. Pete Graham (Robert Downey, Jr.). Drugged and confused, she remembers nothing of what happened after the car accident. To her horror, she learns that her husband Douglas (Charles S. Dutton) was brutally murdered and that she is the primary suspect. While Miranda copes with her new life in the hospital, the ghost uses her body to carry out messages (most noticeably, she carves the words "Not Alone" into Miranda's arm), which leads her former colleagues to believe Miranda is suicidal and is inflicting the wounds on herself.

Alexa

Alexa may refer to:

Alexa Internet

Alexa Internet, Inc. is a California-based company that provides commercial web traffic data and analytics. It is a wholly owned subsidiary of Amazon.com.

Founded as an independent company in 1996, Alexa was acquired by Amazon in 1999. Its toolbar collects data on browsing behavior and transmits them to the Alexa website, where they are stored and analyzed, forming the basis for the company's web traffic reporting. According to its website, Alexa provides traffic data, global rankings and other information on 30 million websites, and as of 2015 its website is visited by over 6.5 million people monthly.

Operations and history

Alexa Internet was founded in April 1996 by American web entrepreneurs Brewster Kahle and Bruce Gilliat. The company's name was chosen in homage to the Library of Alexandria of Ptolemaic Egypt, drawing a parallel between the largest repository of knowledge in the ancient world and the potential of the Internet to become a similar store of knowledge.

Alexa initially offered a toolbar that gave Internet users suggestions on where to go next, based on the traffic patterns of its user community. The company also offered context for each site visited: to whom it was registered, how many pages it had, how many other sites pointed to it, and how frequently it was updated. Alexa's operations grew to include archiving of web pages as they are crawled. This database served as the basis for the creation of the Internet Archive accessible through the Wayback Machine. In 1998, the company donated a copy of the archive, two terabytes in size, to the Library of Congress. Alexa continues to supply the Internet Archive with Web crawls.

Alexa Fluor

The Alexa Fluor family of fluorescent dyes is produced by Molecular Probes, Inc., a wholly owned subsidiary of Life Technologies Corporation. Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology.

The excitation and emission spectra of the Alexa Fluor series cover the visible spectrum and extend into the infrared. The individual members of the family are numbered according roughly to their excitation maxima (in nm).

Alexa Fluor dyes are synthesized through sulfonation of coumarin, rhodamine, xanthene (such as fluorescein), and cyanine dyes. Sulfonation makes Alexa Fluor dyes negatively charged and hydrophilic. Alexa Fluor dyes are generally more stable, brighter, and less pH-sensitive than common dyes (e.g. fluorescein, rhodamine) of comparable excitation and emission, and to some extent the newer cyanine series. However, they are also more expensive. They are patented by Invitrogen (which acquired the company that developed the Alexa Fluor dyes, Molecular Probes).