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Open access restriction at Hook Links (3): outcome (Natural England)
Public Technologies 11 Mar 2025- 1
The AT-hook is a DNA-binding motif present in many proteins, including the high mobility group (HMG) proteins, DNA-binding proteins from plants and hBRG1 protein, a central ATPase of the human switching/sucrose non-fermenting (SWI/SNF) remodeling complex.
This motif consists of a conserved, palindromic, core sequence of proline-arginine-glycine-arginine-proline, although some AT-hooks contain only a single proline in the core sequence. AT-hooks also include a variable number of positively charged lysine and arginine residues on either side of the core sequence. The AT-hook binds to the minor groove of adenine-thymine (AT) rich DNA, hence the AT in the name. The rest of the name derives from a predicted asparagine/aspartate "hook" in the earliest AT-hooks reported in 1990. In 1997 structural studies using NMR determined that a DNA-bound AT-hook adopted a crescent or hook shape around the minor groove of a target DNA strand (pictured at right). HMGA proteins contain three AT-hooks, although some proteins contain as many as 30. The optimal binding sequences for AT-hook proteins are repeats of the form (ATAA)n or (TATT)n, although the optimal binding sequences for the core sequence of the AT-hook are AAAT and AATT.
[Chorus]
You heard what I said (yes sir yes sir!)
You heard what I said (yes sir yes sir!)
I said you heard what I said (yes sir yes sir!)
You heard what I said (yes sir yes sir!)
[Verse 1: White Dawg]
Run game, slang cane, Broward playas mane
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[Verse 2: Dozia Slim]
Open up a can of whoop ass on yo bitch ass
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[Chorus x2]
You heard what I said (yes sir yes sir!)
You heard what I said (yes sir yes sir!)
I said you heard what I said (yes sir yes sir!)
You heard what I said (yes sir yes sir!)
[Bridge x8]
We be some game runners, just some game runners
[Verse 3: White Dawg]
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Some died in the Caddy on some D's that was rolling
[Verse 4: Dozia Slim]
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Drop them 'bos on them hoes fuck em then they dead
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[Chorus x2]
You heard what I said (yes sir yes sir!)
You heard what I said (yes sir yes sir!)
I said you heard what I said (yes sir yes sir!)
You heard what I said (yes sir yes sir!)
[Bridge x8]