Directionality (molecular biology)

Directionality, in molecular biology and biochemistry, is the end-to-end chemical orientation of a single strand of nucleic acid. In a single strand of DNA or RNA, the chemical convention of naming carbon atoms in the nucleotide sugar-ring means that there will be a 5-end, which frequently contains a phosphate group attached to the 5 carbon of the ribose ring, and a 3-end (usually pronounced "five prime end" and "three prime end"), which typically is unmodified from the ribose -OH substituent. In a DNA double helix, the strands run in opposite directions to permit base pairing between them, which is essential for replication or transcription of the encoded information.

Nucleic acids can only be synthesized in vivo in the 5-to-3 direction, as the polymerases that assemble various types of new strands generally rely on the energy produced by breaking nucleoside triphosphate bonds to attach new nucleoside monophosphates to the 3-hydroxyl (-OH) group, via a phosphodiester bond. The relative positions of structures along a strand of nucleic acid, including genes and various protein binding sites, are usually noted as being either upstream (towards the 5-end) or downstream (towards the 3-end). (See also upstream and downstream.)

Podcasts:

PLAYLIST TIME:

Waiting For Love

by: 3T

[Lil' Mo]
Tupac and Lil' Mo, hmm, how gangsta is that?
Hehe.. ooooh-oooh, ooooh-oooh, ooooh-oooh
[Mo keeps harmonizing in the background]
[2Pac]
One two.. to a nigga nature, haha..
No need to cry now, go wipe your tears, be a woman
Why you actin surprised? You saw the bullshit
Comin fake hair, fake nails, fake eyes too
So why you, bound to fuck wit fake guys too
Aint nothin' hard about it why you lookin sad? Shoulda though about it
Say you learned, I truly doubt it
I guess you got a problem with affection, kinda loose with the love
Gettin freaky with the thug niggaz up in the club
Ask to buy you a drink, you holla Dom Perignon
Knowin I'm a cash getter still I, remain calm
Let you chill with me; plus you was smilin 'til the bill miss me
That's what you get for tryin to dick me
Missed me with that "Buy me this, buy me that" syndrome shit
Bitch get a job if you wanna be rich
Gettin mad cause I cursed and I scream I hate'cha
Introduced you to a nigga nature, feel me?
[Chorus: Lil' Mo]
Kissed the girls, made them cry
Thuggin life, and gettin high
Why you gangsta, all the time?
That's a thug's nature
Though sometimes, I can deal with it
I realize, that I'm feelin it
It's a love and hate relationship
but that's a thug's nature
[add to line four of Chorus first time:] "That's a nigga nature"
[2Pac]
I'm probably too nice at first, I let you kiss me where it hurts
Me and you gettin busy, slingin dick in the dirt
Met you at a pool party it was cool to kick it
See us, tounge-kissin, you was truly with it
Little ecstacy, Hennesey, mix with me
Picture me pay for pussy when the dick's for free
Hey now, where my niggaz at? Tell these hoes
Before I pay; I jerk off, word to Moses
Visions of you sittin there sweaty and wet
Pointin to the places that you want me to hit
Give me room all up in the womb, call the cops
Nigga, hittin walls 'til them bastard drop
Label me Makaveli - thug nigga with bite
Livin life like a rock star's Friday night
Make money, get pussy, always keep a pager
Cell phone in the ride to complete my nature now!
[Chorus]
[2Pac]
Haha.. started as a seed from the semen; straight outta papa's nuts
Lustin for creamin - bitches with big butts
Curves make a nigga cry, tits and shit
When I'm locked down beggin you for porno flicks
Sneak weed in, help a nigga pass the time
Put my name tattoo'd so that ass is mine
Tell everybody; 'Pac put it down for good
A local legend through the whole hood, follow me
I got a gun on me, goin for none on the run baby
You know a nigga need some, is my son crazy?
Why I cry, when I be thuggin til I die
Picture a nigga in heaven, high off weed I fly
Got me missin dead homies wishin phonies would die
Hit the weed and hope it get me high; dear God
Understand my ways, livin major
Blessed with a thug's heart.. and a real live nigga nature
[Chorus]
[add to line four of Chorus:] "That's a nigga nature"
[add to line eight of Chorus:] "Cause that's a nigga nature"
[Chorus]
[add to line four of Chorus:] "Hey, just be a nigga nature"
[add to line eight of Chorus:] "Cause that's a nigga nature"
[2Pac] It ain't my fault
[Lil' Mo] Hehe, Q.D., where you be? Ahh
[2Pac] Don't blame me blame my momma, a nigga nature
[Lil' Mo]
QDIII, and Lil' Mo
Tupac, puttin it down fo' sho' ("Cause that's a nigga nature")
I realize, that I'm feelin it
Cause that's a thug nature
Though sometimes I can deal with it
I realize, I'm feelin it
Love and hate, relationship
Cause that's a thug's nature ("Cause that's a nigga nature")
Yeah yeah yeah.. yeah yeah yeah..
Yeahhh yeah.. and that's a thug's nature
Where you at? Holla




Latest News for: 5 to 3

Edit

US man to plead guilty to trying to kill Supreme Court justice

Iosco County News Herald 03 Apr 2025
A California man who flew across the country with a plan to assassinate a conservative US Supreme Court judge will plead guilty to the attempted killing, lawyers said Wednesday in a court filing ... .
Edit

Israel PM says 'dissecting' Gaza to force Hamas to free hostages

Mount Carmel Register 03 Apr 2025
Israeli Prime Minister Benjamin Netanyahu said on Wednesday that the military was "dissecting" the Gaza Strip and seizing territory to pressure Hamas into freeing hostages still held in the territory ... .
Edit

WATCH: As Trump aims to curb mail-in voting, some Illinois lawmakers look to expand it

Mount Carmel Register 03 Apr 2025
(The Center Square) – Despite some pushback from county clerks in the state, some Illinois lawmakers want to control how some aspects of voting are done during elections ... .
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Michigan congressman traveling to Mexico to 'demand' couple's release from prison

Grand Haven Tribune 03 Apr 2025
Another chapter is beginning in the weekslong saga of Christy and Paul Akeo, a Michigan couple behind bars in a maximum security Mexican prison over a timeshare dispute ... .
Edit

GOP lawmaker revives effort to strip MT voters' ability to elect some regulators

Independent Record 03 Apr 2025
A push to remove the ability of Montana voters to elect three-fifths of the Montana Public Service Commission came back to life this week ... .
Edit

WATCH: DOJ asks judge to deny IL’s motion to dismiss migrant sanctuary lawsuit

Iosco County News Herald 03 Apr 2025
(The Center Square) – The U.S. Department of Justice is urging a federal district court judge to deny a motion to dismiss its challenge to Illinois’ migrant sanctuary policies ... .
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Scientists use tech to track toilet breaks to keep Antarctica clean

Iosco County News Herald 03 Apr 2025
They use vehicle tracking and colored dials on its snowmobiles to monitor when and where they have toilet breaks ... .
Edit

Mark Zuckerberg Shells Out $23 Million to Live Closer to Trump

The Daily Beast 03 Apr 2025
Mark Zuckerberg has found a new way of cozying up to President Donald Trump ... The Meta CEO purchased a $23 million, 15,000-square-foot mansion in Washington, D.C.’s high-end Woodland Normanstone neighborhood, according to Politico.
Edit

President Trump to Order 'Reciprocal Tariffs' to Begin at Midnight

BitRSS 03 Apr 2025
Bitcoin retreated to $86,000 in volatile action as the announcements were made ... .
Edit

Letter to the Editor: Thanks to all who helped the Knights of Columbus Tootsie Roll Drive

Craig Daily Press 03 Apr 2025
A special thanks to City Market for allowing us to set up in front of the store.
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Save Up to 20% on Your Phone Plan Thanks to Ultra Mobile’s Spring Sale

CNET 03 Apr 2025
Need to lower your monthly phone bill? Switching to Ultra Mobile could be a wise decision. We'll explain why ... .
Edit

'So obvious I don't need to comment' - Slot disputes decision not to send off Tarkowski

BBC News 03 Apr 2025
Liverpool manager Arne Slot disputes the decision to only award a yellow card to Everton's James Tarkovski for his tackle on Alexis Mac Allister in his side's 1-0 Premier League win in the Merseyside derby at Anfield ... .
Edit

£10 added to UK energy bills to support wood-burning firm Drax

The Times/The Sunday Times 03 Apr 2025
British households paid around £10 each, on average, on their energy bills last year to support a controversial wood-burning power station ...Drax power station to get £2bn subsidy despite green-energy row.
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