Five-prime cap

In molecular biology, the five-prime cap (5′ cap) is a specially altered nucleotide on the 5′ end of some eukaryotic primary transcripts such as precursor messenger RNA. This process, known as mRNA capping, is highly regulated and vital in the creation of stable and mature messenger RNA able to undergo translation during protein synthesis. Mitochondrial and chloroplast mRNA are not capped.

Structure

In eukaryotes, the 5′ cap (cap-0), found on the 5′ end of an mRNA molecule, consists of a guanine nucleotide connected to mRNA via an unusual 5′ to 5′ triphosphate linkage. This guanosine is methylated on the 7 position directly after capping in vivo by a methyltransferase. It is referred to as a 7-methylguanylate cap, abbreviated m⁷G.

In multicellular eukaryotes and some viruses, further modifications exist, including the methylation of the 2′ hydroxy-groups of the first 2 ribose sugars of the 5′ end of the mRNA. cap-1 has a methylated 2'-hydroxy group on the first ribose sugar, while cap-2 has methylated 2'-hydroxy groups on the first two ribose sugars, shown on the right. The 5′ cap is chemically similar to the 3′ end of an RNA molecule (the 5′ carbon of the cap ribose is bonded, and the 3′ unbonded). This provides significant resistance to 5′ exonucleases.

Raw image format

A camera raw image file contains minimally processed data from the image sensor of either a digital camera, image scanner, or motion picture film scanner. Raw files are named so because they are not yet processed and therefore are not ready to be printed or edited with a bitmap graphics editor. Normally, the image is processed by a raw converter in a wide-gamut internal colorspace where precise adjustments can be made before conversion to a "positive" file format such as TIFF or JPEG for storage, printing, or further manipulation, which often encodes the image in a device-dependent colorspace. There are dozens, if not hundreds, of raw formats in use by different models of digital equipment (like cameras or film scanners).

Rationale

Raw image files are sometimes called digital negatives, as they fulfill the same role as negatives in film photography: that is, the negative is not directly usable as an image, but has all of the information needed to create an image. Likewise, the process of converting a raw image file into a viewable format is sometimes called developing a raw image, by analogy with the film development process used to convert photographic film into viewable prints. The selection of the final choice of image rendering is part of the process of white balancing and color grading.

Cap (crown)

The cap of a crown is the cap which fills the inner space of a modern crown. While ancient crowns contained no cap, from mediaeval times it became traditional to fill the circlet with a cap of velvet or other such cloth, with a base of ermine.

While the precise reason for the inclusion of a cap is unknown, two reasons are often given:

Not all crowns contained cloth caps. Some caps were metallic and heavily jewelled.

Pileus (mycology)

The pileus is the technical name for the cap, or cap-like part, of a basidiocarp or ascocarp (fungal fruiting body) that supports a spore-bearing surface, the hymenium. The hymenium (hymenophore) may consist of lamellae, tubes, or teeth, on the underside of the pileus. A pileus is characteristic of agarics, boletes, some polypores, tooth fungi, and some ascomycetes.

Classification

Pilei can be formed in various shapes, and the shapes can change over the course of the developmental cycle of a fungus. The most familiar pileus shape is hemispherical or convex. Convex pilei often continue to expand as they mature until they become flat. Many well-known species have a convex pileus, including the button mushroom, various Amanita species and boletes.

Some, such as the parasol mushroom, have distinct bosses or umbos and are described as umbonate. An umbo is a knobby protrusion at the center of the cap. Some fungi, such as chanterelles have a funnel- or trumpet-shaped appearance. In these cases the pileus is termed infundibuliform.