Amplify-and-forward (AF) relay multiple-input multiple-output (MIMO) orthogonal frequency division multiplexing (OFDM) systems can achieve high data rate and high quality communications. On the other hand, it has to estimate all channels between the source-relay and relay-destination nodes in the destination node. In MIMO/OFDM systems, high time resolution carrier interferometry (HTRCI) has been proposed to achieve an accurate channel estimation (CE) with a small number of pilot signals. However, since it has many interferences, an accurate CE is not obtained and the system performance is degraded in AF relay MIMO/OFDM systems. Therefore, in this paper, we propose the complex HTRCI (C-HTRCI) pilot signal and the enhanced minimum mean square error (E-MMSE) equalization to achieve an accurate CE and to improve the system performance for AF relay MIMO/OFDM systems.