Alan Ryder
CURRENT CORE RESEARCH AREAS:
Biopharmaceutical & protein analysis: Development of novel rapid, quantitative analytical methods for use in the BioPharma sector. Most focus at the moment is on the use of polarized Excitation Emission Matrix (pEEM) spectroscopy for the analysis of proteins and vaccines.
Another core focus is the development of rapid spectroscopic methods for cell culture media analysis. This research was initially part of a collaborative project, the Centre for BioAnalytical Science (CBAS), with DCU and Bristol Myers-Squibb (2005-09). The project involves the study of all aspects of the biopharma production process from raw staring materials, to media, to process samples, and up to finished product.
Advanced Fluorescence Microscopy: A second component of the research involves the application of advanced fluorescence microscopy techniques to measuring dynamic biological and chemical processes at surfaces, and in living cells. The involves the use of methods such as Fluorescence Lifetime Imaging Microscopy (FLIM), Fluorescence Correlation Spectroscopy (FCS), Fluorescence Cross-Correlation Spectroscopy (FCCS), and Total Internal Reflection Fluorescence Microscopy (TIRFM). This aspect of my research programme encompasses a wide range of collaborators and is continually growing. More details at: www.universityofgalway.ie/nanoscale/instrumentation/microscopy/
Biomaterials: This encompasses three different yet interlinked projects. First, the development of fluorescence based methods for measuring surface and bulk polarity of polymers, which is also linked with studies to develop methods for measuring drug elution rates from biomedical polymers and the behaviour of liposome drug delivery systems. The second core project is the development of ultra-sensitive methods for measuring the deposition and the conformation of proteins on surfaces. The final strand involves the development of FLIM instrumentation for wide field imaging of biofilms.
Raman spectroscopy and Chemometrics: Development of quantitative methods of analysis for pharmaceutical, biopharmaceutical, forensic, and law enforcement applications. Development of novel measurement methods for nanoparticles and nanosuspensions using Raman.
Petroleum fluid analysis: Development of instrumentation and analysis methods for crude oil characterisation and quantification. Study of the fundamental processes governing petroleum fluorescence. Study of petroleum bearing fluid inclusions. More details at: www.universityofgalway.ie/nanoscale/researchprojects/petroleum/
Spectroscopic Facilities & Instrumentation: This area encompasses all the research interests and involves both the development of new instrumentation, modifying of existing systems for specific applications, and the provision of state-of-the-art facilities. This has represented a considerable effort since 1997 and we have >2M€+ of instrumentation in the NBL. These include:
• FLIM/FCS: Two confocal microscopes for materials and life science applications using FCS/FCCS for the study of proteins in complex systems. Several EMCCD cameras are also available for ultra-low light imaging. A third system (Thorlabs VCM100) was delivered in May 2008.
• Total Internal Reflection Fluorescence Microscopy (TIRFM), installed in 2008 with a top-spec EMCCD camera. Used for protein-surface studies.
• Raman spectroscopy: Three Raman systems, one handheld (2009), one workstation (2012), and one for bulk materials analysis (2005).
• Fluorescence spectroscopy: Includes 8 steady-state spectrometers and multiple lifetime systems.
• General Analytical: Extensive array of equipment including HPLC, DLS, UV-visible, refractometer, water activity meter, DLS, Osmometer, microwave plasma Atomic Emission, XRF.
Phone: 353-91-492943
Address: Nanoscale Biophotonics Laboratory,
University of Galway,
University Road,
Galway, H91TK33, Ireland.
Biopharmaceutical & protein analysis: Development of novel rapid, quantitative analytical methods for use in the BioPharma sector. Most focus at the moment is on the use of polarized Excitation Emission Matrix (pEEM) spectroscopy for the analysis of proteins and vaccines.
Another core focus is the development of rapid spectroscopic methods for cell culture media analysis. This research was initially part of a collaborative project, the Centre for BioAnalytical Science (CBAS), with DCU and Bristol Myers-Squibb (2005-09). The project involves the study of all aspects of the biopharma production process from raw staring materials, to media, to process samples, and up to finished product.
Advanced Fluorescence Microscopy: A second component of the research involves the application of advanced fluorescence microscopy techniques to measuring dynamic biological and chemical processes at surfaces, and in living cells. The involves the use of methods such as Fluorescence Lifetime Imaging Microscopy (FLIM), Fluorescence Correlation Spectroscopy (FCS), Fluorescence Cross-Correlation Spectroscopy (FCCS), and Total Internal Reflection Fluorescence Microscopy (TIRFM). This aspect of my research programme encompasses a wide range of collaborators and is continually growing. More details at: www.universityofgalway.ie/nanoscale/instrumentation/microscopy/
Biomaterials: This encompasses three different yet interlinked projects. First, the development of fluorescence based methods for measuring surface and bulk polarity of polymers, which is also linked with studies to develop methods for measuring drug elution rates from biomedical polymers and the behaviour of liposome drug delivery systems. The second core project is the development of ultra-sensitive methods for measuring the deposition and the conformation of proteins on surfaces. The final strand involves the development of FLIM instrumentation for wide field imaging of biofilms.
Raman spectroscopy and Chemometrics: Development of quantitative methods of analysis for pharmaceutical, biopharmaceutical, forensic, and law enforcement applications. Development of novel measurement methods for nanoparticles and nanosuspensions using Raman.
Petroleum fluid analysis: Development of instrumentation and analysis methods for crude oil characterisation and quantification. Study of the fundamental processes governing petroleum fluorescence. Study of petroleum bearing fluid inclusions. More details at: www.universityofgalway.ie/nanoscale/researchprojects/petroleum/
Spectroscopic Facilities & Instrumentation: This area encompasses all the research interests and involves both the development of new instrumentation, modifying of existing systems for specific applications, and the provision of state-of-the-art facilities. This has represented a considerable effort since 1997 and we have >2M€+ of instrumentation in the NBL. These include:
• FLIM/FCS: Two confocal microscopes for materials and life science applications using FCS/FCCS for the study of proteins in complex systems. Several EMCCD cameras are also available for ultra-low light imaging. A third system (Thorlabs VCM100) was delivered in May 2008.
• Total Internal Reflection Fluorescence Microscopy (TIRFM), installed in 2008 with a top-spec EMCCD camera. Used for protein-surface studies.
• Raman spectroscopy: Three Raman systems, one handheld (2009), one workstation (2012), and one for bulk materials analysis (2005).
• Fluorescence spectroscopy: Includes 8 steady-state spectrometers and multiple lifetime systems.
• General Analytical: Extensive array of equipment including HPLC, DLS, UV-visible, refractometer, water activity meter, DLS, Osmometer, microwave plasma Atomic Emission, XRF.
Phone: 353-91-492943
Address: Nanoscale Biophotonics Laboratory,
University of Galway,
University Road,
Galway, H91TK33, Ireland.
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