Salik Nazki

Indian Journal of Animal Research Published Volume 58 Issue 5 (May 2024) In vitro Antimicrobial, Antibiofilm and Antiquorum Sensing Activity of Indian Rhododendron (Melastoma malabathricum) against Clinical Isolates of Escherichia coli and Staphylococcus aureus Honeysmita Das, A.K. Samanta, Sanjeev Kumar, P. Roychoudhury, Kalyan Sarma, Fatema Akter, P.K. Subudhi, T.K. Dutta doi: 10.18805/IJAR.B-4415 Cite article:- Das Honeysmita, Samanta A.K., Kumar Sanjeev, Roychoudhury P., Sarma Kalyan, Akter Fatema, Subudhi P.K., Dutta T.K. (2024). In vitro Antimicrobial, Antibiofilm and Antiquorum Sensing Activity of Indian Rhododendron (Melastoma malabathricum) against Clinical Isolates of Escherichia coli and Staphylococcus aureus . Indian Journal of Animal Research. 58(5): 841-846. doi: 10.18805/IJAR.B-4415. ABSTRACT Background: Development and persistence of multidrug resistant (MDR) bacteria is considered to be one of the biggest threats to public health worldwide. Development of new antimicrobial agents and alternatives to the conventional antimicrobial agents to control the menace of AMR is the need of the hour. Plants based products can be effectively explored as potential antimicrobial, antibiofilm and antiquorum sensing agents against major bacterial pathogens of human and animals. This present study was conducted to explore the antimicrobial, antibiofilm and antiquorum sensing activity of aqueous and methanol extracts of leaf, flower, fruit and stem of Melastoma malabathricum against clinical isolates of Staphylococcus aureus and Escherichia coli. Methods: E. coli and S. aureus were isolated and identified from diarrhoeic pigs and poultry and mastitic milk of cattle of Mizoram, respectively. Leaf, flower, fruit and stem of M. malabathricum were collected from Mizoram and extracted by methanol and aqueous solvents. The antimicrobial activity and MIC was determined by using well diffusion method and 96 wells microtiter plate method, respectively. Antibiofilm activity of plant extracts was determined in 96 well tissue culture plate. Antiquorum sensing activity was determined by disc diffusion method. Result: Methanol leaf extract exhibited antimicrobial activity against E. coli but not against S. aureus with 18 mm and 6 mm zone of inhibition at 200 mg/mL and 12.5 mg/mL, respectively. Methanol flower extract showed antimicrobial activity against S. aureus but not against E. coli with 14 mm and 6 mm zone of inhibitions at 200 mg/ml and 12.5 mg/mL, respectively. Similarly, the aqueous leaf extract showed antimicrobial activity against S. aureus but not against E. coli with 12 mm and 6 mm zone of inhibition at 200 mg/mL and 100 mg/mL, respectively. KEYWORDS Antibiofim, Antimicrobial, Antiquorum Sensing, Melastoma Malabathricum https://lnkd.in/gEXJZ_NC

Indian Journal of Animal Research Published Volume 58 Issue 6 (JUNE 2024) Detection and Molecular Characterization of VP1 Gene of Chicken Infectious Anaemia Virus in Suspected Layer Birds Tejashree Kulkarni, Mrunalini Pawade, Uma Tumlam, Rahul Kolhe, Prajwalini Mehere, Dushyant Muglikar, Prashant Mhase, Sunil Kapgate doi: 10.18805/IJAR.B-5037 Cite article:- Kulkarni Tejashree, Pawade Mrunalini, Tumlam Uma, Kolhe Rahul, Mehere Prajwalini, Muglikar Dushyant, Mhase Prashant, Kapgate Sunil (2024). Detection and Molecular Characterization of VP1 Gene of Chicken Infectious Anaemia Virus in Suspected Layer Birds . Indian Journal of Animal Research. 58(6): 1022-1026. doi: 10.18805/IJAR.B-5037. ABSTRACT Background: Chicken Infectious Anaemia Virus (CIAV) is one of the emerging pathogen of the poultry. The Genus of this virus is Gyrovirus and the family Anelloviridae. Viral DNA contains three open reading frames (ORFs) encoding for three proteins VP1, VP2 and VP3. VP1 protein is major structural protein and is a capsid protein having major role in growth and spread of the virus. Present study was aimed for molecular detection of VP1 gene of the CIA virus in the samples collected from the outbreaks occurred at four different district of Maharashtra. Methods: Total 50 samples comprising of Spleen, bone marrow, thymus and liver, were collected. DNA extracted from those samples was used for PCR amplification targeting, VP1 gene. Selected positive purified PCR products of VP1 were subjected for Nucleotide sequencing and Phylogenetic analysis. Results: Total out of 50 pooled samples, 10 samples were positive for VP1 gene i.e. 20 %. Sequencing of coding region of four CAV positive samples was conducted. Genetic analysis showed three isolates had high similarity with Del-Ros vaccine strainfrom USA ; while one isolate shared close similarity to attenuated cloned isolate 10 of Cux-1 strain. The data also indicated Q139 and Q144 and Q394 amino acids substitutions among the VP1. Phylogenetic analysis of the sequenced viruses based on either the coding nucleotide sequence or VP1 coding sequence, suggested the circulation of III genotypes in Maharashtra. KEYWORDS Amino Acid Analysis, Chicken Infectious Anaemia, PCR, Phylogenetic Analysis, VP1 Gene https://lnkd.in/gBp_MPZh

Legume Research Published Volume 47 Issue 8 (AUGUST 2024) Amplified Fragment Length Polymorphism based Genetic Diversity of Tropical Legume: Lablab purpureus var. typicus Vishwajeet Singh, Nitin Wahi, Gunjan Garg, Anshu Singh doi10.18805/LR-4814 Cite article:- Singh Vishwajeet, Wahi Nitin, Garg Gunjan, Singh Anshu (2024). Amplified Fragment Length Polymorphism based Genetic Diversity of Tropical Legume: Lablab purpureus var. typicus . Legume Research. 47(8): 1266-1273. doi: 10.18805/LR-4814. ABSTRACT Background: Lablab purpureus L. is a legume appropriate to grow in tropical environment as it is adaptable to a broad temperature spectrum, rainfall and altitudes. It develops well under humid and warm conditions at temperatures ranging between 18oC and 30oC. It is a drought hardy crop grown in humid and semi-arid regions. The species is propagated by seed and can be sown alone or intercropped; planted near hedges or near other less leafy plants to climb on. Lablab purpureus L. has a flowering response of short-day, with early and late flowering types. Some landraces flower after sowing as early as 55 days. It is predominantly self pollinated although some out crossing is known to occur. Methods: In this study, Lablab purpureus L. genetic diversity were analyzed utilizing AFLP markers on ten Indian Lablab purpureus L. accessions at Department of Botany, Bundelkhand University, Jhansi during 2019-20. Five primers set were used for checking the diversity in Lablab purpureus L. and the marker performance were measured by using 4 parameters i.e. PIC, Mean resolving Power, Resolving Power and MI. Result: A total of 52 bands were generated, of which 45 bands (88.46%) were polymorphic which varies from 6 (M-CAT/E-ACT ) to 13 (M-CAC/E-ACC, M-CAC/E-ACT) with mean of 9.00 bands per primer. The PIC value ranges between 0.55 and 0.76 with a mean value of 0.64. The average value of matrix index is 3.04. Higher the MI, better the marker is. The value of RP ranges between 2.60 and 7.80 with a mean value of 4.68. In the UPGMA dendrogram, the 10 accessions were separated into two main clusters with all the primers used. In this study, we have shown that molecular markers can be used successfully to understand the genetic structure of the accessions of Lablab purpureus L. and identified a series of sequence-specific markers that potentially could be used in Lablab purpureus L. genetic mapping analyses. Since Lablab purpureus L. has been considered as a promising crop because of its wide spectrum of adaptability to different ecological conditions than other legumes, participatory evaluation at an early breeding stage could shape the variety being developed to increase the productivity and other traits as well as for utilization as food. KEYWORDS Aflp, Genetic diversity, Lablab purpureus l, Marker, Polymorphic https://lnkd.in/gzw55Huu