Abstract
Rapid sequence analysis of specific PCR products was used to accurately deduce emm types corresponding to the majority of the known group A streptococcal (GAS) M serotypes. The study involved 95 M type reference GAS strains and a survey of 74 recent clinical isolates. A high percentage of agreement between M type serology and the previously published 5' sequences of the emm genes of M type reference strains was noted. The 5' sequences for six established M protein genes--the emm-32, emm-34, emm-38, emm-40, emm-42, and emm-71 genes--were determined to supplement the existing emm sequence database. Rapid sequence analysis differentiated serologically M-nontypeable strains and was used to establish the probable.
MeSH terms
-
Amino Acid Sequence
-
Antigens, Bacterial*
-
Bacterial Outer Membrane Proteins*
-
Bacterial Proteins / genetics*
-
Bacterial Typing Techniques*
-
Carrier Proteins*
-
Databases, Factual
-
Evaluation Studies as Topic
-
Genes, Bacterial*
-
Humans
-
Molecular Sequence Data
-
Polymerase Chain Reaction / methods*
-
Reference Standards
-
Sequence Homology, Amino Acid
-
Serotyping
-
Streptococcus pyogenes / classification*
-
Streptococcus pyogenes / genetics*
-
Streptococcus pyogenes / isolation & purification
Substances
-
Antigens, Bacterial
-
Bacterial Outer Membrane Proteins
-
Bacterial Proteins
-
Carrier Proteins
-
streptococcal M protein
Associated data
-
GENBANK/M11338
-
GENBANK/M19031
-
GENBANK/M20374
-
GENBANK/S82057
-
GENBANK/U11595
-
GENBANK/U11932
-
GENBANK/U11934
-
GENBANK/U11935
-
GENBANK/U11937
-
GENBANK/U11938
-
GENBANK/U11940
-
GENBANK/U11941
-
GENBANK/U11942
-
GENBANK/U11943
-
GENBANK/U11944
-
GENBANK/U11945
-
GENBANK/U11946
-
GENBANK/U11948
-
GENBANK/U11951
-
GENBANK/U11952
-
GENBANK/U11953
-
GENBANK/U11955
-
GENBANK/U11958
-
GENBANK/U11968
-
GENBANK/U11970
-
GENBANK/U11997
-
GENBANK/U12002
-
GENBANK/U12005
-
GENBANK/X15198