Genetic characterization of porcine epidemic diarrhea virus in Korea from 1998 to 2013

Arch Virol. 2015 Apr;160(4):1055-64. doi: 10.1007/s00705-015-2353-y. Epub 2015 Feb 10.

Abstract

The number of porcine epidemic diarrhea (PED) cases has increased over the past 20 years in Korea, with a major outbreak in 2013. A total of 27 Korean strains from 1998 to 2013 were analyzed (excluding the noncoding regions) and divided into two groups for comparison of the spike (S), ORF3, envelope (E), membrane (M), and nucleocapsid (N) genes with those of reference strains, vaccine strains, and previously identified strains based on phylogenetic analysis. Analysis of the selection patterns of PEDV isolated in Korea indicated positive selection of nine nonsynonymous sites in the S and N proteins and negative selection at 97 sites for all of the proteins. Interestingly, eight nonsynonymous mutations in S showed no significant pattern change over the 15-year period, and one of eight mutation sites was found only in IC05TK, GN05DJ, and KNU0802 in the epidemic years 2005 and 2008. These eight mutations were also present during the epidemic years in China. Furthermore, of the signs of positive selection in the S protein, the conservative substitutions were more frequent than radical substitutions in PEDVs, suggesting that the evolution of Korean strains has been slow. Serological cross-reactivity was detected between three field PEDVs and two vaccine strains, with different serum neutralization titers. In conclusion, although Korean PEDVs have been evolving slowly, their diverse antigenicity and genetics imply that multilateral efforts to prevent future PED outbreaks are required.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Coronavirus Infections / veterinary*
  • Coronavirus Infections / virology
  • Diarrhea / veterinary*
  • Diarrhea / virology
  • Genetic Variation
  • Molecular Sequence Data
  • Open Reading Frames
  • Phylogeny
  • Porcine epidemic diarrhea virus / classification
  • Porcine epidemic diarrhea virus / genetics*
  • Porcine epidemic diarrhea virus / isolation & purification*
  • Republic of Korea
  • Swine
  • Swine Diseases / virology*
  • Viral Proteins / genetics

Substances

  • Viral Proteins