Abstract
A new multiplex PCR assay was developed to separate the four major Listeria monocytogenes serovars isolated from food and patients (1/2a, 1/2b, 1/2c, and 4b) into distinct groups. The PCR test, which constitutes a rapid and practical alternative to laborious classical serotyping, was successfully evaluated with 222 Listeria strains.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Base Sequence
-
DNA Primers
-
DNA, Bacterial / genetics
-
DNA, Bacterial / isolation & purification
-
Electrophoresis, Agar Gel
-
Food Microbiology
-
Humans
-
Listeria / classification
-
Listeria / genetics
-
Listeria / isolation & purification
-
Listeria monocytogenes / classification*
-
Listeria monocytogenes / genetics
-
Listeria monocytogenes / isolation & purification*
-
Listeriosis / diagnosis
-
Listeriosis / microbiology
-
Polymerase Chain Reaction / methods
-
Reproducibility of Results
-
Serotyping / methods
Substances
-
DNA Primers
-
DNA, Bacterial