Abstract
We have investigated the role of interferon regulatory factor-1 (IRF-1), an interferon-gamma (IFN-gamma) inducible transcriptional activator, on major histocompatibility complex (MHC) class I gene transcription. IRF-1 alone is sufficient to trans-activate both transfected and endogenous class I genes and the effect of IRF-1 appears to be direct and sequence-specific. These data suggest that IRF-1 is involved in the IFN-gamma mediated induction of MHC class I expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cell Line
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Cloning, Molecular
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DNA-Binding Proteins / physiology*
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Flow Cytometry
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Gene Expression Regulation*
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Genes, Regulator / physiology
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HeLa Cells
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Histocompatibility Antigens Class I / biosynthesis*
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Humans
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Interferon Regulatory Factor-1
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Molecular Sequence Data
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Phosphoproteins / physiology*
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Plasmids
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Promoter Regions, Genetic / physiology
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RNA / analysis
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Trans-Activators / physiology
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Transcription, Genetic / physiology
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Transcriptional Activation
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Transfection
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Up-Regulation / physiology
Substances
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DNA-Binding Proteins
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Histocompatibility Antigens Class I
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IRF1 protein, human
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Interferon Regulatory Factor-1
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Phosphoproteins
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Trans-Activators
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RNA