restriction analysis

restriction analysis

the analysis of DNA molecules by the use of RESTRICTION ENZYMES. The DNA is cleaved with the enzyme(s) and the fragments obtained are generally separated by GEL ELECTROPHORESIS. The pattern of restriction fragments produced can be used for various purposes:
  1. (a) to detect fragments of a specific gene using a nucleic acid PROBE;
  2. (b) to detect the presence of mutations (see RESTRICTION FRAGMENT LENGTH POLYMORPHISM);
  3. (c) to compare DNA from different individuals (see FINGERPRINTING);
  4. (d) to construct physical maps, called restriction maps, of DNA molecules, that indicate the position of restriction sites in the molecules.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
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Positive clones were screened (restriction analysis) by isolation of recombinant plasmid following (Thermo scientific Miniprep Kit) manufacturer recommendations and results were visualized on 1% agarose gel.
SOLUBLE EXPRESSION OF BACILLUS LICHENIFORMIS ATCC 27811 [alpha]-AMYLASE AND CHARACHTERIZATION OF PURIFIED RECOMBINANT ENZYME
In order to establish the dissimilarity between strains we conducted a restriction analysis with PacI and PmeI (Table 2).
Genetic Diversity in Pseudomonas syringae pv. maculicola Strains
The positive clone after restriction analysis was utilized for DNA sequencing (Sanger et al., 1977).
Refolding of Misfolded Inclusion Bodies of Recombinant [alpha]-Amylase: Characterization of Cobalt Activated Thermostable [alpha]-Amylase from Geobacillus SBS-4S
In addition various molecular characterization studies were also carried on different species, cultivars and accessions of Pistacia including RAPD (Kafkas and Perl-Treves, 2001; Kafkas and Perl- Treves, 2002; Katsiotis et al., 2003; Golan-Goldhirsh et al., 2004), AFLP (Katsiotis et al., 2003; Golan-Goldhirsh et al., 2004; Kafkas, 2006; Karimi et al., 2009), ISSR (Kafkas, 2006), SSR, SRAP (Talebi et al., 2012), IRAP (Ghaemmaghami et al., 2013) and restriction analysis of chloroplast fragments (Parfitt and Badenes, 1997) in order to resolve the phylogenetic relationships.
Retrotransposon Marker Systems as a Tool to Analyze Molecular Diversity of Mediterranean Pistacia Species
Three restriction enzymes ( Hha I , BstU I, and Mbo I) were used in restriction analysis.
Rapid Identification of Mycobacterium Leprae by Polymerase Chain Reaction-restriction Fragment Length Polymorphism Analysis of the Heat Shock Protein 65 Gene from Skin Specimens
The primers were synthetized by Bioneer Corporation, South Korea, and all other reagents used in PCR and restriction analysis were purchased by Fermentas, Lithuania, part of Thermo Fisher Scientific.
Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach/Identificacao de especies toxigenicas de Aspergillus isoladas da dieta de cabras leiteiras utilizando uma abordagem polifasica
The isolates from lungs, kidney, heart, intestine, liver, and bone marrow all harbored the same virulence-associated factors (iucD, colV, iss, mat, fimC, ompA, traT crl, csgA vgrG, and hcp), yielded the same band pattern in amplified ribosomal DNA restriction analysis, and were allocated to the E coli Reference Collection group B1.
Avian Dis.: Characterization of extraintestinal Escherichia coli isolated from a peacock (Pavo cristatus) with colisepticemia
The restriction analysis by PstI of the positive PCR samples further subcategorized into two genotypes (Fig.
Genotyping of MSP3[beta] gene in Indian Plasmodium vivax

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