As a result, induced motor neurons expressed [beta]3-tubulins (
Tubb2a and Tubb2b), Map2, synapsins (Syn1 and Syn2), synaptophysin (Syp), and synaptotagmins (Syt1, Syt4, Syt13, and Syt16) and had such electrophysiological characteristics of normal motor neurons as resting potential of -49.5 mV (SEM 5.6, N = 6), presence of active Na-K channels that were blocked by tetrodotoxin, and the ability to generate a characteristic neuronal action potential.
Each sample was profiled in duplicates for expression of
TUBB2A, [8] TBP, GUSB, GAPDH, EMP2, VIM, VEGFA, PLAU, TWIST1, TP53, TOP2A, SCGB2A2, SATB1, RAD51, PTPRC, PTEN, PIK3CA, PGR, PARP1, MYC, MUC1, MTOR, ABCC1, MET, KRT19, KRAS, KIT,MKI67, IGF1R, IBSP, FLT1, ESR1, ERBB2, EPCAM, EGFR, CTSD, CDH1, CD44, CD24, AURAK, ALDH1A1, AKT2, ADAM17, ACTB (Actin fi), PPIA, and B2M.