Online-Ressource | |
Verfasst von: | Fogeron, Marie-Laure [VerfasserIn] |
Paul, David [VerfasserIn] | |
Jirasko, Vlastimil [VerfasserIn] | |
Bartenschlager, Ralf [VerfasserIn] | |
Titel: | Functional expression, purification, characterization, and membrane reconstitution of non-structural protein 2 from hepatitis C virus |
Verf.angabe: | Marie-Laure Fogeron, David Paul, Vlastimil Jirasko, Roland Montserret, Denis Lacabanne, Jennifer Molle, Aurélie Badillo, Célia Boukadida, Sonia Georgeault, Philippe Roingeard, Annette Martin, Ralf Bartenschlager, François Penin, Anja Böckmann |
E-Jahr: | 2015 |
Jahr: | 29 August 2015 |
Umfang: | 6 S. |
Teil: | volume:116 |
year:2015 | |
pages:1-6 | |
extent:6 | |
Fussnoten: | Gesehen am 03.08.2021 |
Titel Quelle: | Enthalten in: Protein expression and purification |
Ort Quelle: | Amsterdam [u.a.] : Elsevier, 1990 |
Jahr Quelle: | 2015 |
Band/Heft Quelle: | 116(2015), Seite 1-6 |
Abstract: | Non-structural protein 2 (NS2) of the hepatitis C virus (HCV) is an integral membrane protein that contains a cysteine protease and that plays a central organizing role in assembly of infectious progeny virions. While the crystal structure of the protease domain has been solved, the NS2 full-length form remains biochemically and structurally uncharacterized because recombinant NS2 could not be prepared in sufficient quantities from cell-based systems. We show here that functional NS2 in the context of the NS2-NS3pro precursor protein, ensuring NS2-NS3 cleavage, can be efficiently expressed by using a wheat germ cell-free expression system. In this same system, we subsequently successfully produce and purify milligram amounts of a detergent-solubilized form of full-length NS2 exhibiting the expected secondary structure content. Furthermore, immuno-electron microscopy analyses of reconstituted proteoliposomes demonstrate NS2 association with model membranes. |
DOI: | doi:10.1016/j.pep.2015.08.027 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt. Volltext ; Verlag: https://doi.org/10.1016/j.pep.2015.08.027 |
Volltext: https://www.sciencedirect.com/science/article/pii/S1046592815300474 | |
DOI: https://doi.org/10.1016/j.pep.2015.08.027 | |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Cell-free protein expression |
Hepatitis C virus | |
Lipid reconstitution | |
Membrane protein | |
Non-structural protein 2 | |
K10plus-PPN: | 1765315433 |
Verknüpfungen: | → Zeitschrift |