Pandoraea thiooxydans DSM 25325(T) is a thiosulfate-oxidizing bacterium isolated from rhizosphere... more Pandoraea thiooxydans DSM 25325(T) is a thiosulfate-oxidizing bacterium isolated from rhizosphere soils of a sesame plant. Here, we present the first complete genome of P. thiooxydans DSM 25325(T). Several genes involved in thiosulfate oxidation and biodegradation of aromatic compounds were identified.
Serratia multitudinisentens RB-25(T) (=DSM 28811(T) =LMG 28304(T)) is a newly proposed type strai... more Serratia multitudinisentens RB-25(T) (=DSM 28811(T) =LMG 28304(T)) is a newly proposed type strain in the genus of Serratia isolated from a municipal landfill site. Here, we present the complete genome of S. multitudinisentens RB-25(T) which contains a complete chitinase operon and other chitin and N-acetylglucosamine utilisation enzymes. To our knowledge, this is the first report of the complete genome sequence of this novel isolate and its chitinase gene discovery.
Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly fo... more Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found in various habitats in Antarctica. Quorum quenching (QQ) is the disruption of bacterial cell-to-cell communication (known as quorum sensing), which has previously been described in mesophilic bacteria. This study demonstrated the QQ activity of a psychrotolerant strain, Planococcus versutus strain L10.15(T), isolated from a soil sample obtained near an elephant seal wallow in Antarctica. Whole genome analysis of this bacterial strain revealed the presence of an N-acyl homoserine lactonase, an enzyme that hydrolyzes the ester bond of the homoserine lactone of N-acyl homoserine lactone (AHLs). Heterologous gene expression in E. coli confirmed its functions for hydrolysis of AHLs, and the gene was designated as aidP (autoinducer degrading gene from Planococcus sp.). The low temperature activity of this enzyme suggested that it is a novel and uncharacterized class of AHL lactonase. This ...
International journal of systematic and evolutionary microbiology, Jan 12, 2016
A taxonomic study was performed on a novel Gram-staining-positive, cocci-shaped, orange-pigmented... more A taxonomic study was performed on a novel Gram-staining-positive, cocci-shaped, orange-pigmented motile bacterium, designated strain L10.15T. The organism was isolated from a soil sample collected on Lagoon Island (close to Adelaide Island, western Antarctic Peninsula) using a quorum quenching enrichment medium. Growth occurred at 4-30 °C, pH 6-11, and at moderately high salinity (0-15 %), with optimal growth at 26 °C, at pH 7-8 and 6% NaCl. The 16S rRNA gene sequence analysis showed that strain L10.15T belonged to the genus Planococcus and was closely related to P. halocryophilus Or1T (99.3 %), P. donghaensis JH 1T (99.0 %), P. antarticus DSM 14505T (98.3 %), P. plakortidis AS/ASP6 (II)T (97.6 %), P. maritimus TF-9T (97.5 %), P. salinavum ISL-6 T (97.5 %), and P. kocurii NCIMB 629T (97.5 %). However, the ANI-MUMmer (ANIm) analysis showed low genomic relatedness values of 71.1-81.7% to the type strains of these closely related species of the genus Planococcus. The principal fatty a...
International journal of systematic and evolutionary microbiology, Jan 15, 2016
Phylogenetic and taxonomic characterization was performed for bacterium RB-25T which was isolated... more Phylogenetic and taxonomic characterization was performed for bacterium RB-25T which was isolated from a soil sample collected in a former municipal landfill site in Puchong, Malaysia. Growth occurs at 20-37 °C at pH 5-8 and does not grow in the presence of 9 % (w/v) NaCl or higher. The principal fatty acids were C16 : 0, C18:1 ω 7c, and summed feature 3 (C16:1 ω 7c and/or iso-C15:0 2-OH). Ubiquinone-8 was the only isoprenoid quinone detected. Polar lipid analysis revealed presence of phospholipid, phosphoaminolipid, phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. DNA G+C content was 50.9 %. The phylogenetic analysis based on 16S rRNA gene sequence showed that strain RB-25T formed a distinct lineage within the Enterobacteriaceae family of Gammaproteobacteria class. It exhibited low level of 16S rRNA gene sequence similarity with its phylogenetic neighbours Pantoea rwandensis LMG 26275T (96.6 %), Rahnella aquatilis CIP 78.65T (96.5 %), Pectobacterium b...
Pseudomonas sp. strain L10.10 (=DSM 101070) is a psychrotolerant bacterium which was isolated fro... more Pseudomonas sp. strain L10.10 (=DSM 101070) is a psychrotolerant bacterium which was isolated from Lagoon Island, Antarctica. Analysis of its complete genome sequence indicates its possible role as a plant-growth promoting bacterium, including nitrogen-fixing ability and indole acetic acid (IAA)-producing trait, with additional suggestion of plant disease prevention attributes via hydrogen cyanide production.
Planococcus rifietoensis M8(T) (=DSM 15069(T)=ATCC BAA-790(T)) is a halotolerant bacterium with p... more Planococcus rifietoensis M8(T) (=DSM 15069(T)=ATCC BAA-790(T)) is a halotolerant bacterium with potential plant growth promoting properties isolated from an algal mat collected from a sulfurous spring in Campania (Italy). This paper presents the first complete genome of P. rifietoensis M8(T). Genes coding for various potentially plant growth promoting properties were identified within its genome.
Pandoraea thiooxydans DSM 25325(T) is a thiosulfate-oxidizing bacterium isolated from rhizosphere... more Pandoraea thiooxydans DSM 25325(T) is a thiosulfate-oxidizing bacterium isolated from rhizosphere soils of a sesame plant. Here, we present the first complete genome of P. thiooxydans DSM 25325(T). Several genes involved in thiosulfate oxidation and biodegradation of aromatic compounds were identified.
Staphylococcus saprophyticusstrain SU8 was isolated from a pristine water source in Malaysia and ... more Staphylococcus saprophyticusstrain SU8 was isolated from a pristine water source in Malaysia and it exhibited degradation ofN-hexanoylhomoserine lactone. Here we report the draft genome sequence ofS. saprophyticusstrain SU8 to further understand its quorum quenching abilities.
Pandoraea pnomenusa RB-38 is a bacterium isolated from a former sanitary landfill site. Here, we ... more Pandoraea pnomenusa RB-38 is a bacterium isolated from a former sanitary landfill site. Here, we present the complete genome of P. pnomenusa RB38 in which an oxalate utilization pathway was identified. The genome analysis suggested the potential of this strain as an effective biocontrol agent against oxalate-producing phytopathogens.
Pandoraea thiooxydans DSM 25325(T) is a thiosulfate-oxidizing bacterium isolated from rhizosphere... more Pandoraea thiooxydans DSM 25325(T) is a thiosulfate-oxidizing bacterium isolated from rhizosphere soils of a sesame plant. Here, we present the first complete genome of P. thiooxydans DSM 25325(T). Several genes involved in thiosulfate oxidation and biodegradation of aromatic compounds were identified.
Serratia multitudinisentens RB-25(T) (=DSM 28811(T) =LMG 28304(T)) is a newly proposed type strai... more Serratia multitudinisentens RB-25(T) (=DSM 28811(T) =LMG 28304(T)) is a newly proposed type strain in the genus of Serratia isolated from a municipal landfill site. Here, we present the complete genome of S. multitudinisentens RB-25(T) which contains a complete chitinase operon and other chitin and N-acetylglucosamine utilisation enzymes. To our knowledge, this is the first report of the complete genome sequence of this novel isolate and its chitinase gene discovery.
Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly fo... more Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found in various habitats in Antarctica. Quorum quenching (QQ) is the disruption of bacterial cell-to-cell communication (known as quorum sensing), which has previously been described in mesophilic bacteria. This study demonstrated the QQ activity of a psychrotolerant strain, Planococcus versutus strain L10.15(T), isolated from a soil sample obtained near an elephant seal wallow in Antarctica. Whole genome analysis of this bacterial strain revealed the presence of an N-acyl homoserine lactonase, an enzyme that hydrolyzes the ester bond of the homoserine lactone of N-acyl homoserine lactone (AHLs). Heterologous gene expression in E. coli confirmed its functions for hydrolysis of AHLs, and the gene was designated as aidP (autoinducer degrading gene from Planococcus sp.). The low temperature activity of this enzyme suggested that it is a novel and uncharacterized class of AHL lactonase. This ...
International journal of systematic and evolutionary microbiology, Jan 12, 2016
A taxonomic study was performed on a novel Gram-staining-positive, cocci-shaped, orange-pigmented... more A taxonomic study was performed on a novel Gram-staining-positive, cocci-shaped, orange-pigmented motile bacterium, designated strain L10.15T. The organism was isolated from a soil sample collected on Lagoon Island (close to Adelaide Island, western Antarctic Peninsula) using a quorum quenching enrichment medium. Growth occurred at 4-30 °C, pH 6-11, and at moderately high salinity (0-15 %), with optimal growth at 26 °C, at pH 7-8 and 6% NaCl. The 16S rRNA gene sequence analysis showed that strain L10.15T belonged to the genus Planococcus and was closely related to P. halocryophilus Or1T (99.3 %), P. donghaensis JH 1T (99.0 %), P. antarticus DSM 14505T (98.3 %), P. plakortidis AS/ASP6 (II)T (97.6 %), P. maritimus TF-9T (97.5 %), P. salinavum ISL-6 T (97.5 %), and P. kocurii NCIMB 629T (97.5 %). However, the ANI-MUMmer (ANIm) analysis showed low genomic relatedness values of 71.1-81.7% to the type strains of these closely related species of the genus Planococcus. The principal fatty a...
International journal of systematic and evolutionary microbiology, Jan 15, 2016
Phylogenetic and taxonomic characterization was performed for bacterium RB-25T which was isolated... more Phylogenetic and taxonomic characterization was performed for bacterium RB-25T which was isolated from a soil sample collected in a former municipal landfill site in Puchong, Malaysia. Growth occurs at 20-37 °C at pH 5-8 and does not grow in the presence of 9 % (w/v) NaCl or higher. The principal fatty acids were C16 : 0, C18:1 ω 7c, and summed feature 3 (C16:1 ω 7c and/or iso-C15:0 2-OH). Ubiquinone-8 was the only isoprenoid quinone detected. Polar lipid analysis revealed presence of phospholipid, phosphoaminolipid, phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. DNA G+C content was 50.9 %. The phylogenetic analysis based on 16S rRNA gene sequence showed that strain RB-25T formed a distinct lineage within the Enterobacteriaceae family of Gammaproteobacteria class. It exhibited low level of 16S rRNA gene sequence similarity with its phylogenetic neighbours Pantoea rwandensis LMG 26275T (96.6 %), Rahnella aquatilis CIP 78.65T (96.5 %), Pectobacterium b...
Pseudomonas sp. strain L10.10 (=DSM 101070) is a psychrotolerant bacterium which was isolated fro... more Pseudomonas sp. strain L10.10 (=DSM 101070) is a psychrotolerant bacterium which was isolated from Lagoon Island, Antarctica. Analysis of its complete genome sequence indicates its possible role as a plant-growth promoting bacterium, including nitrogen-fixing ability and indole acetic acid (IAA)-producing trait, with additional suggestion of plant disease prevention attributes via hydrogen cyanide production.
Planococcus rifietoensis M8(T) (=DSM 15069(T)=ATCC BAA-790(T)) is a halotolerant bacterium with p... more Planococcus rifietoensis M8(T) (=DSM 15069(T)=ATCC BAA-790(T)) is a halotolerant bacterium with potential plant growth promoting properties isolated from an algal mat collected from a sulfurous spring in Campania (Italy). This paper presents the first complete genome of P. rifietoensis M8(T). Genes coding for various potentially plant growth promoting properties were identified within its genome.
Pandoraea thiooxydans DSM 25325(T) is a thiosulfate-oxidizing bacterium isolated from rhizosphere... more Pandoraea thiooxydans DSM 25325(T) is a thiosulfate-oxidizing bacterium isolated from rhizosphere soils of a sesame plant. Here, we present the first complete genome of P. thiooxydans DSM 25325(T). Several genes involved in thiosulfate oxidation and biodegradation of aromatic compounds were identified.
Staphylococcus saprophyticusstrain SU8 was isolated from a pristine water source in Malaysia and ... more Staphylococcus saprophyticusstrain SU8 was isolated from a pristine water source in Malaysia and it exhibited degradation ofN-hexanoylhomoserine lactone. Here we report the draft genome sequence ofS. saprophyticusstrain SU8 to further understand its quorum quenching abilities.
Pandoraea pnomenusa RB-38 is a bacterium isolated from a former sanitary landfill site. Here, we ... more Pandoraea pnomenusa RB-38 is a bacterium isolated from a former sanitary landfill site. Here, we present the complete genome of P. pnomenusa RB38 in which an oxalate utilization pathway was identified. The genome analysis suggested the potential of this strain as an effective biocontrol agent against oxalate-producing phytopathogens.
Uploads
Papers by Wai-fong Yin