Papers by Vladimir Mirsky
Biological Chemistry, Aug 1, 1998
As more relatives of the low density lipoprotein receptor (LDLR) are discovered, defining their m... more As more relatives of the low density lipoprotein receptor (LDLR) are discovered, defining their minimal binding domain(s) becomes a challenge. Here we have chosen the multifunctional chicken oocyte receptor for yolk deposition (termed LR8), and the pan-receptor ligand, receptor associated protein (RAP), as model systems to characterize a minireceptor using the phage display approach. Displayed fragments derived from the entire 819 residue LR8 molecule, followed by selection via panning on RAP, led to the definition of an 80 residue stretch LR8 minireceptor. It contains 12 cysteines, and represents parts of the second, the entire third, and parts of the fourth, of the eight clustered 'ligand binding repeats' in LR8; only two of the eight stretches of negatively charged residues of LR8, i.e., EDGSDE and DSGEDEE, are present. The latter sequence is reminiscent of that in the fifth repeat of the human LDLR, thought to be most critical for interaction with positive charge clusters in ligands. Baculovirus-mediated expression of the soluble minireceptor in insect cells showed it to fold as a monomer, and sulfhydryl-reduction-sensitive interaction with RAP was demonstrated for immobilized as well as soluble minireceptor. Furthermore, the LR8-derived minireceptor provided a RAP-responsive surface when covalently coupled to the surface of a gold electrode. In addition to its use in defining minimal binding domains, the phage display approach provides powerful tools for dissection, and consequently, manipulation, of the function of receptors so as to direct their binding activity toward ligands of diagnostic and/or therapeutic interest.
Analytical Chemistry
... display obvious advantages in electrochemical experiments and in single electrochemical senso... more ... display obvious advantages in electrochemical experiments and in single electrochemical sensors, the present ... Considering a simplified equivalent circuit of the electrode/water interface (Figure 2), one ... An optimized capacitive chemical sensor should have a maximum (R l ) for ...
Bulletin of Experimental Biology and Medicine
Solid State Ionics
We report on the optical ozone sensitivity of a polyaniline (PANI) film prepared on the inner wal... more We report on the optical ozone sensitivity of a polyaniline (PANI) film prepared on the inner wall of a polystyrene cuvette by chemical oxidation of aniline from aqueous solution. The PANI film showed changes of optical absorbance in the presence of 50–100 ppm ozone in air at room temperature. The absorbance change was largest at wavelengths in the range of 500–800 nm. The absorbance changes brought about by ozone was partially reversible after removal of ozone from the atmosphere. These results suggest that PANI is a potential material for optical ozone detection.
Sensors and Actuators B: Chemical, 2015
Sensors and Actuators B: Chemical, 2015
MIRSKY:ARTIFICAL RECEPTOR O-BK, 2010
TrAC Trends in Analytical Chemistry, 2002
Combinatorial Methods for Chemical and Biological Sensors, 2009
Conductive polymers are established materials for development of chemical and biological sensors.... more Conductive polymers are established materials for development of chemical and biological sensors. Properties of these polymers are influenced by a number of different physical and chemical factors. Application of combinatorial and high-throughput techniques to development and optimization of chemo and biosensors is reviewed. Methods for addressable synthesis of conductive polymers and protocols for comprehensive description of chemosensitive properties are discussed.
... for Anions 5.5 Receptors for Neutral Substrates 5.6 Conclusion References Chapter 6: Boronic ... more ... for Anions 5.5 Receptors for Neutral Substrates 5.6 Conclusion References Chapter 6: Boronic Acid-Based Receptors and Chemosensors 6.1 Introduction 6.2 De Novo Design 6.3 Combinatorial Approaches 6.4 Template-Directed Synthesis Acknowledgment References ...
The Fourth International Conference on Advanced Semiconductor Devices and Microsystem, 2002
A novel automated set-up for combinatorial formation of conductive polymer films and investigatio... more A novel automated set-up for combinatorial formation of conductive polymer films and investigation of their electrical properties is outlined. The set-up provides controlled electropolymerization on platinum or gold electrodes and consists of a dosing station, an electronic block for control and measurement and an electrode array. Conditions of electropolymerizations and measurements can be programmed by user and they are automatically, executed. Polymer layers are created on a electrode array with 96 single interdigital electrodes specially designed for four-point measurements to exclude contact effects. The first results obtained for electropolymerization of aniline are presented.
Fresenius' Journal of Analytical Chemistry, 2000
ABSTRACT A simple technique for the calibration of mercury vapor chemosensors is described. It is... more ABSTRACT A simple technique for the calibration of mercury vapor chemosensors is described. It is based on a reductive deposition of a well-defined quantity of mercury (0) onto gold wire followed by thermal evaporation of the mercury into the sensor cell. The quantity of mercury on the gold wire was measured electrochemically by oxidation before and after heating, as well as after storage under different conditions. It is shown that the technique can be used for calibrated injections of nanogram quantities of mercury. The approach was applied to test the performance of ultrasensitive mercury chemoresistors.
Thin film electrode chips on silicon substrate have been realized and utilized in development of ... more Thin film electrode chips on silicon substrate have been realized and utilized in development of different chemo- and biosensors. These affinity sensors are based on capacitive transducing and their general structure is thin film Au/alkanethiol/receptor. Several applications of thin film gold electrodes in capacitive sensors are presented
Comprehensive Analytical Chemistry, 2007
ABSTRACT 15.1 OBJECTIVES (a) Preparation of chemical sensor for mercury vapor; (b) electrochem-ic... more ABSTRACT 15.1 OBJECTIVES (a) Preparation of chemical sensor for mercury vapor; (b) electrochem-ical calibration procedure, and (c) application of the sensor for meas-urements of mercury vapor. The sensor is based on high affinity of gold to mercury and on chemo-resistive properties of ultrathin gold layers: adsorption of mercury leads to increase in the surface resistance [1,2]. However, this effect is not selective: similar changes are caused by adsorption of water vapor and sulfuric compounds. The use of monomolecular layer of alkylthiols as a filter excludes this interference completely [3]. Sensor calibration is performed by thermoinjection of nanogram-amount of mercury quanti-tatively deposited by electrochemical reduction [4]. 15.2 MATERIALS AND INSTRUMENTS Reduction of mercury on gold wires used for calibration is performed with potentiostat VoltaLab32 (Radiometer, France) at room tempera-ture ($221C), with cell volume of 40 ml. The electrode assembly con-sisted of a gold wire electrode (99.99% purity, from Chempur, Germany) as a working electrode (50 mm  5 cm), a platinum wire as an auxiliary electrode, and a saturated Ag/AgCl electrode with a double salt bridge filled by 0.1 M KNO 3 as a reference electrode. The mechan-ical stability of the gold wire electrode was provided by a glass support (Fig. 15.1). Thermoinjection of Hg addition in the calibration cell is based on electrical heating by applying 2 V voltage to the amalgamated gold wire electrode. The applied power is sufficient to release Hg from the amalgamated form avoiding the melting of Au wire. Comprehensive Analytical Chemistry 49 Alegret and Merkoc (Eds) Volume 49 ISSN: 0166-526X DOI: 10.1016/S0166-526X(06)49058-9 r 2007 Elsevier B.V. All rights reserved.
Comprehensive Analytical Chemistry, 2007
Springer Series on Chemical Sensors and Biosensors, 2004
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Papers by Vladimir Mirsky