Valeri Beck

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Seit 2014Technischer Leiter, PhysikerProgrammierung und Fertigung von Elektronikbaugruppen, Entwicklung und Fertigung von kundenspezifischen Einzelteilen aus Carbon. Forschung und Entwicklung im Bereich der neuen Plasmatechnologie.Alfa-Carbon® Beck, Berlin

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Papers by Valeri Beck

Method and apparatus for forming a lipid bilayer membrane

Predictions of planets for 586 exosystems and a method for predicting planets in multi-planetary exosystems

We have applied a model of planetary system formation in the field of a standing sound wave (the ... more We have applied a model of planetary system formation in the field of a standing sound wave (the ‘SSW-Model’) to the prediction of planets in 586 multi-planetary exosystems (384 biplanetary, 127 tri-planetary and 75 exosystems with four or more confirmed planets). We have verified our predictions using transit-like events from the NASA Threshold-Crossing Event (TCE) catalogue, finding that more than 80% of these events are included in our list of exoplanet predictions. We describe a method for predicting the periods and semi-major axes of additional planets for exosystems with two or more confirmed planets. Our method can significantly facilitate targeted searches for planets in exosystems, enabling the detection of many new exoplanets, including those located in the habitable zones.

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A sound nebula: the origin of the Solar System in the field of a standing sound wave

According to the planetary origin conceptual model proposed in this paper, the protosun centre of... more According to the planetary origin conceptual model proposed in this paper, the protosun centre of the pre-solar nebula exploded, resulting in a shock wave that passed through it and then returned to the centre, generating a new explosion and shock wave. Recurrent explosions in the nebula resulted in a spherical standing sound wave, whose antinodes concentrated dust into rotating rings that transformed into planets. The extremely small angular momentum of the Sun and the tilt of its equatorial plane were caused by the asymmetry of the first, most powerful explosion. Differences between inner and outer planets are explained by the migration of solid matter, while the Oort cloud is explained by the division of the pre-solar nebula into a spherical internal nebula and an expanding spherical shell of gas. The proposed conceptual model can also explain the origin and evolution of exoplanetary systems and may be of use in searching for new planets.

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Techniques for forming a lipid bilayer membrane

A new automated technique for the reconstitution of hydrophobic proteins into planar bilayer membranes. Studies of human recombinant uncoupling protein 1

by Elena Pohl and Valeri Beck

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2006

Electrophysiological characterisation of the vast number of annotated channel and transport prote... more Electrophysiological characterisation of the vast number of annotated channel and transport proteins in the postgenomic era would be greatly facilitated by the introduction of rapid and robust methods for the functional incorporation of membrane proteins into defined lipid bilayers. Here, we describe an automated technique for reconstitution of membrane proteins into lipid bilayer membranes, which substantially reduces both the reconstitution time and the amount of protein required for the membrane formation. The method allows the investigation of single protein channels as well as insertion of multiple copies (approximately 10(7)) into a single bilayer. Despite a comparatively large membrane area (up to 300 microm diameter), the high stability of the membrane permits the application of transmembrane voltages up to 300 mV. This feature is especially important for studies of inner membrane mitochondrial proteins, since they act at potentials up to approximately 200 mV under physiological conditions. It is a combination of these advantages that enables the detailed investigation of the minuscule single protein conductances typical for proton transporters. We have applied the new technique for the reconstitution and electrophysiological characterisation of human recombinant uncoupling protein 1, hUCP1, that has been overexpressed in E. coli and purified from inclusion bodies. We demonstrate that hUCP1 activity in the presence of fatty acids is comparable to the activity of UCP1 isolated from brown adipose tissue.

Role of the Transmembrane Potential in the Membrane Proton Leak

by Elena Pohl and Valeri Beck

Biophysical Journal, 2010

The molecular mechanism responsible for the regulation of the mitochondrial membrane proton condu... more The molecular mechanism responsible for the regulation of the mitochondrial membrane proton conductance (G) is not clearly understood. This study investigates the role of the transmembrane potential (DeltaPsim) using planar membranes, reconstituted with purified uncoupling proteins (UCP1 and UCP2) and/or unsaturated FA. We show that high DeltaPsim (similar to DeltaPsim in mitochondrial State IV) significantly activates the protonophoric function of UCPs in the presence of FA. The proton conductance increases nonlinearly with DeltaPsim. The application of DeltaPsim up to 220 mV leads to the overriding of the protein inhibition at a constant ATP concentration. Both, the exposure of FA-containing bilayers to high DeltaPsim and the increase of FA membrane concentration bring about the significant exponential Gm increase, implying the contribution of FA in proton leak. Quantitative analysis of the energy barrier for the transport of FA anions in the presence and absence of protein suggests that FA- remain exposed to membrane lipids while crossing the UCP-containing membrane. We believe this study shows that UCPs and FA decrease DeltaPsim more effectively if it is sufficiently high. Thus, the tight regulation of proton conductance and/or FA concentration by DeltaPsim may be key in mitochondrial respiration and metabolism.