Wound healing may be defined as a loss or breaking of cellular and anatomic or functional continu... more Wound healing may be defined as a loss or breaking of cellular and anatomic or functional continuity of living tissues. In the present investigation, wound healing activity of Curcuma longa with Oleum olivae was carried out. The progressive decrease in the wound area was monitored every 3 days tracing the wound margin on a tracing paper and assessed using group paper. The wound contraction was measured as percentage reduction in the wound area in each animal and the damaged skin prior to treatment and regenerated skin after treatment was examined. The wound applied with Curcuma longa powder with Oleum olivae showed a faster contraction when compared to normal control.
Isolation of lignin degrading microorganisms from cow dung, soil compost, and paper pulp effluent... more Isolation of lignin degrading microorganisms from cow dung, soil compost, and paper pulp effluent was carried out in the present investigation. Totally 9 isolates of lignin degrading microbes were isolated using minimal salt media containing lignin (MSM-L) and lignolytic activities were preliminary screened by testing against methylene blue indicator dye containing LB medium. Eight microbes showed positive results and the predominant isolates were identified as Pseudomonas sp. The findings indicated that Pseudomonas sp. has significant potential for use in the applications for the treatment of lignin degradation and lignin related environment pollutants.
Lead toxicity in soil has become a global issue due to increase and high demand of safety food. I... more Lead toxicity in soil has become a global issue due to increase and high demand of safety food. In the present investigation an attempted were made on the toxicity effect of lead acetate on the germinal growth of Black Gram seeds. The seeds were influenced to germinate under various concentration of Lead Acetate (Control, 10 mM, 20 mM, 30 mM, 40 mM and 50 mM). The seed germination root length parameters and total chlorophyll estimation were analyzed. Increasing in the concentration of Lead causes drastic reduction in the seed germination and also wide influenced in the decreased level of chlorophyll content. The result reflects that the Lead is toxic to crop growth and development.
Objective: The aim of the present study was to evaluate the hypolipidemic efficacy of the seeds o... more Objective: The aim of the present study was to evaluate the hypolipidemic efficacy of the seeds of two common varieties of Momordica charantia extract (MCSEt1 and MCSEt2) in streptozotocin induced diabetic rats. Methods: Streptozotocin (55mg/kg body weight) was used for induction of diabetes in rats. MCSEt1 and MCSEt2 extracts (150 mg/kg body weight) was administered orally for 30 days to experimental rats. The fatty acid composition of liver and kidney were analyzed by gas chromatography. Results: Oral administration of MCSEt1 and MCSEt2 extracts resulted in a significant reduction in the levels of cholesterol, phospholipids, triglycerides and free fatty acids in plasma and tissues of STZinduced diabetic rats. The altered fatty acid composition in liver and kidney were restored by the treatment. Glibenclamide was used as a reference drug to compare the efficacy of the seeds extract. Though both the varieties exhibited significant lipid lowering properties, the efficacy was pronounc...
Artocarpus hirsutus Lam.,(Wild jack) belonging to the family Moraceae a large evergreen tree up t... more Artocarpus hirsutus Lam.,(Wild jack) belonging to the family Moraceae a large evergreen tree up to 70m height, found up to an altitude of 1200M in evergreen India. The wood is straight blackish brown in color; it is very strong tree and has main advantage of lightness. It is used for the treatment of ulcers, diarrhea and pimples. The present study includes Pharmacognostical studies of the bark of Artocarpus hirsutus Lam.
Role of humic acids (HA) in plant tissue culture as plant growth regulators has been reviewed. Hu... more Role of humic acids (HA) in plant tissue culture as plant growth regulators has been reviewed. Humic acid is a principal component of humic substances, which are the most important organic constituents of the soil (humus). Humus is defined as the organic matter in soil, a mixture of partially and totally humified substances. Leonardite is the best source for humic acid and it is extracted by using alkali solutions like NaOH/KOH. Humic acid plays a vital role in the plant tissue culture as growth hormone for in vitro propagation of many plant seedlings. This review provides the role of HA in plant tissue culture, field trails and other uses in agriculture. This practice reduces the usage of growth regulators with 2/4 and 1/4 strength MS culture media. This review also helps researchers to carry out experiments with various economically important crops in cost effective manner.
The present study was aimed to evaluate the hypoglycemic efficacy in an aqueous extract of seeds ... more The present study was aimed to evaluate the hypoglycemic efficacy in an aqueous extract of seeds of two varieties, namely a country and a hybrid variety of Momordica charantia (MCSEt1 and MCSEt2) respectively in streptozotocin (STZ) induced diabetic rats. STZ-induced diabetic rats were treated with aqueous extracts of MCSEt1 and t2 for a period of 30 days. MCSEt1 and t2 extract treatment to diabetic rats resulted in a significant reduction in blood glucose, glycosylated hemoglobin, lactate dehydrogenase, glucose-6-phosphatase, fructose-1,6-bisphosphatase and glycogen phosphorylase, and a concomitant increase in the levels of hemoglobin, glycogen and activities of hexokinase and glycogen synthase. These results clearly show the antidiabetic properties of Momordica charantia. Both the varieties showed safe and significant hypoglycemic effects which were more pronounced in MCSEt1 compared to MCSEt2 and glibenclamide.
Basic phytochemical screening from the aerial parts of Anisochilus carnosus revealed the presence... more Basic phytochemical screening from the aerial parts of Anisochilus carnosus revealed the presence of alkaloids, flavonoids, glycosides, tannins, steroids in high activity, carbohydrates, oils and fats in less activity. The goal of this study is to identify and characterize the bioactive compounds present in this valuable medicinal plant. For the identification and isolation of these phytochemical compounds, different parts of the plant were subjected to extraction of with different solvents namely ethanol, chloroform, acetone and diethyl ether, where ethanolic leaf and flower extracts proved to have high activity. Ethanolic extract of the leaves of this plant showed greater content of these phytochemicals. Further, the dried leaves of the plant are then subjected to extraction with n-hexane and chromatography was performed. The steroid compounds were isolated from the n-hexane extract of the leaves from Anisochilus carnosus. Based on the spectral evidence, 1 H-NMR and 13 C-NMR, stru...
Humic acid is a principal component of Humic substances, which are the major organic constituents... more Humic acid is a principal component of Humic substances, which are the major organic constituents of soil (humus), peat, coal, many upland streams, dystrophic lakes, and ocean water. It is produced by biodegradation of dead organic matter. Leonardite act as a best source for humic acid.The antioxidant activity of coal extracted humic acid (CHA) on in vitro propagated Musa accuminata was examined with commercially available Keradix (KHA), Humic Rooting (BHA). The Polyphenols was found to be higher in native plants of about 146.2µg/ml whereas in CHA it is 54µg/ml. Similarly the amount of protein in BHA is found to be 7µg/ml and CHA is 2.65µg/ml. The catalase activity was found to be higher in BHA is 0.09 U/mg compared to CHA which is estimated as 0.048 U/mg. GST was measured and it was found to be significantly increased in CHA of 582.99 U/mg compared with other commercially available products. The Ascorbic acid Peroxidase activity was higher in Keradix of about 725.31 U/mg APX. The S...
Genetic variations, if any, in Musa accuminata exposed to Humic acid ; Coal extracted Humic acid ... more Genetic variations, if any, in Musa accuminata exposed to Humic acid ; Coal extracted Humic acid (CHA), Biochar Humic acid (BHA) and Commercially available Keradix (KHA) was evaluated using Random amplified polymorphic DNA (RAPD), Simple Sequence Repeat (SSR) and inter simple sequence repeat (ISSR). In this study tissue culture of Musa accuminata enhanced with various concentrations ranging from 0.1 – 0.5% of Humic acid was carried out in MS medium. The isolated DNA amplified with 2 ISSR, 2 SSR and 2 RAPD primers. The PCR based markers such as RAPD and ISSR were useful techniques for detection of variation atgenetic level. The present protocol used for large scale production of Humic acid enhanced Musa accuminata produce genetically stable plants and was proved that it can be used without any problem.
Humic Acid is the most important constituent of a completely decomposed organic matter. Leonardit... more Humic Acid is the most important constituent of a completely decomposed organic matter. Leonardite is found to be the best source for Humic Acid and it is extracted by using alkali solution. Humic Acid scientifically proves to be a free radical scavenger. The study determines that Humic Acid (HA) is used to enhance stress tolerance in plant tissue culture. The induction of the antioxidant system [polyphenol, Catalase (CAT), Superoxide dismutase (SOD),Ascorbate peroxidase (APX) and Glutathione S Transferase (GST)] and also the elevation of the content of the enzymes show that the noted changes provoked the treatment of Humic Acid to Musa accuminata. Humic acid exposed plants (GWO4 –HU) were found to exhibit only 55 µg of GAE g/dw while native plant (GWO4 – NA) exhibited 147.5 µg of GAE g/dw. The increase of polyphenols in the native may be due to the exposure of roots to the heavy metals that are present in activated charcoal used in rooting medium or due to metal ions in water used....
Though vanadium complexes mimic the action of insulin, owing to their toxicity, research is still... more Though vanadium complexes mimic the action of insulin, owing to their toxicity, research is still in progress for a new vanadium complex with maximum efficacy at low concentration and without any side effects. A novel macrocyclic binuclear oxovanadium complex was synthesized, its composition and structure were confirmed by spectral studies and its efficacy was studied in streptozotocin-induced diabetic rats over a period of 30 days. The oral administration of the complex normalizes the blood glucose level in the diabetic rats and also maintains normoglycemia after a glucose load. The biochemical studies revealed that the complex is not toxic to the system. The nontoxic nature of this complex may be due to the presence of the vanadyl ions in an intact form. The study highlights the nontoxic and hypoglycemic effects of the new macrocyclic binuclear oxovanadium complex.
Medicinal herbs used in indigenous medicines for the management of diabetes mellitus contain both... more Medicinal herbs used in indigenous medicines for the management of diabetes mellitus contain both organic and inorganic constituents. Some of these inorganic trace elements possess antidiabetic properties, which accounts for the activity of medicinal herbs. The aim of this study was to analyze the inorganic trace elements present in Eugenia jambolana seeds and to evaluate the hypoglycemic activity of the inorganic part of E. jambolana seeds on streptozotocin-induced diabetes. The seeds of E. jambolana seeds were reduced to ash and the inorganic elements present were assayed. The hypoglycemic efficacy of the inorganic part was tested by the glucose tolerance test on streptozotocin-induced diabetes. Elements such as zinc, chromium, vanadium, potassium, and sodium, possessing hypoglycemic activity, were present in the seed. The E. jambolana seed ash-treated diabetic rats exhibited normoglycemia and better glucose tolerance. The conclusion that the inorganic constituents might play a important role in the antidiabetic nature E. jambolana seeds was reached.
Wound healing may be defined as a loss or breaking of cellular and anatomic or functional continu... more Wound healing may be defined as a loss or breaking of cellular and anatomic or functional continuity of living tissues. In the present investigation, wound healing activity of Curcuma longa with Oleum olivae was carried out. The progressive decrease in the wound area was monitored every 3 days tracing the wound margin on a tracing paper and assessed using group paper. The wound contraction was measured as percentage reduction in the wound area in each animal and the damaged skin prior to treatment and regenerated skin after treatment was examined. The wound applied with Curcuma longa powder with Oleum olivae showed a faster contraction when compared to normal control.
Isolation of lignin degrading microorganisms from cow dung, soil compost, and paper pulp effluent... more Isolation of lignin degrading microorganisms from cow dung, soil compost, and paper pulp effluent was carried out in the present investigation. Totally 9 isolates of lignin degrading microbes were isolated using minimal salt media containing lignin (MSM-L) and lignolytic activities were preliminary screened by testing against methylene blue indicator dye containing LB medium. Eight microbes showed positive results and the predominant isolates were identified as Pseudomonas sp. The findings indicated that Pseudomonas sp. has significant potential for use in the applications for the treatment of lignin degradation and lignin related environment pollutants.
Lead toxicity in soil has become a global issue due to increase and high demand of safety food. I... more Lead toxicity in soil has become a global issue due to increase and high demand of safety food. In the present investigation an attempted were made on the toxicity effect of lead acetate on the germinal growth of Black Gram seeds. The seeds were influenced to germinate under various concentration of Lead Acetate (Control, 10 mM, 20 mM, 30 mM, 40 mM and 50 mM). The seed germination root length parameters and total chlorophyll estimation were analyzed. Increasing in the concentration of Lead causes drastic reduction in the seed germination and also wide influenced in the decreased level of chlorophyll content. The result reflects that the Lead is toxic to crop growth and development.
Objective: The aim of the present study was to evaluate the hypolipidemic efficacy of the seeds o... more Objective: The aim of the present study was to evaluate the hypolipidemic efficacy of the seeds of two common varieties of Momordica charantia extract (MCSEt1 and MCSEt2) in streptozotocin induced diabetic rats. Methods: Streptozotocin (55mg/kg body weight) was used for induction of diabetes in rats. MCSEt1 and MCSEt2 extracts (150 mg/kg body weight) was administered orally for 30 days to experimental rats. The fatty acid composition of liver and kidney were analyzed by gas chromatography. Results: Oral administration of MCSEt1 and MCSEt2 extracts resulted in a significant reduction in the levels of cholesterol, phospholipids, triglycerides and free fatty acids in plasma and tissues of STZinduced diabetic rats. The altered fatty acid composition in liver and kidney were restored by the treatment. Glibenclamide was used as a reference drug to compare the efficacy of the seeds extract. Though both the varieties exhibited significant lipid lowering properties, the efficacy was pronounc...
Artocarpus hirsutus Lam.,(Wild jack) belonging to the family Moraceae a large evergreen tree up t... more Artocarpus hirsutus Lam.,(Wild jack) belonging to the family Moraceae a large evergreen tree up to 70m height, found up to an altitude of 1200M in evergreen India. The wood is straight blackish brown in color; it is very strong tree and has main advantage of lightness. It is used for the treatment of ulcers, diarrhea and pimples. The present study includes Pharmacognostical studies of the bark of Artocarpus hirsutus Lam.
Role of humic acids (HA) in plant tissue culture as plant growth regulators has been reviewed. Hu... more Role of humic acids (HA) in plant tissue culture as plant growth regulators has been reviewed. Humic acid is a principal component of humic substances, which are the most important organic constituents of the soil (humus). Humus is defined as the organic matter in soil, a mixture of partially and totally humified substances. Leonardite is the best source for humic acid and it is extracted by using alkali solutions like NaOH/KOH. Humic acid plays a vital role in the plant tissue culture as growth hormone for in vitro propagation of many plant seedlings. This review provides the role of HA in plant tissue culture, field trails and other uses in agriculture. This practice reduces the usage of growth regulators with 2/4 and 1/4 strength MS culture media. This review also helps researchers to carry out experiments with various economically important crops in cost effective manner.
The present study was aimed to evaluate the hypoglycemic efficacy in an aqueous extract of seeds ... more The present study was aimed to evaluate the hypoglycemic efficacy in an aqueous extract of seeds of two varieties, namely a country and a hybrid variety of Momordica charantia (MCSEt1 and MCSEt2) respectively in streptozotocin (STZ) induced diabetic rats. STZ-induced diabetic rats were treated with aqueous extracts of MCSEt1 and t2 for a period of 30 days. MCSEt1 and t2 extract treatment to diabetic rats resulted in a significant reduction in blood glucose, glycosylated hemoglobin, lactate dehydrogenase, glucose-6-phosphatase, fructose-1,6-bisphosphatase and glycogen phosphorylase, and a concomitant increase in the levels of hemoglobin, glycogen and activities of hexokinase and glycogen synthase. These results clearly show the antidiabetic properties of Momordica charantia. Both the varieties showed safe and significant hypoglycemic effects which were more pronounced in MCSEt1 compared to MCSEt2 and glibenclamide.
Basic phytochemical screening from the aerial parts of Anisochilus carnosus revealed the presence... more Basic phytochemical screening from the aerial parts of Anisochilus carnosus revealed the presence of alkaloids, flavonoids, glycosides, tannins, steroids in high activity, carbohydrates, oils and fats in less activity. The goal of this study is to identify and characterize the bioactive compounds present in this valuable medicinal plant. For the identification and isolation of these phytochemical compounds, different parts of the plant were subjected to extraction of with different solvents namely ethanol, chloroform, acetone and diethyl ether, where ethanolic leaf and flower extracts proved to have high activity. Ethanolic extract of the leaves of this plant showed greater content of these phytochemicals. Further, the dried leaves of the plant are then subjected to extraction with n-hexane and chromatography was performed. The steroid compounds were isolated from the n-hexane extract of the leaves from Anisochilus carnosus. Based on the spectral evidence, 1 H-NMR and 13 C-NMR, stru...
Humic acid is a principal component of Humic substances, which are the major organic constituents... more Humic acid is a principal component of Humic substances, which are the major organic constituents of soil (humus), peat, coal, many upland streams, dystrophic lakes, and ocean water. It is produced by biodegradation of dead organic matter. Leonardite act as a best source for humic acid.The antioxidant activity of coal extracted humic acid (CHA) on in vitro propagated Musa accuminata was examined with commercially available Keradix (KHA), Humic Rooting (BHA). The Polyphenols was found to be higher in native plants of about 146.2µg/ml whereas in CHA it is 54µg/ml. Similarly the amount of protein in BHA is found to be 7µg/ml and CHA is 2.65µg/ml. The catalase activity was found to be higher in BHA is 0.09 U/mg compared to CHA which is estimated as 0.048 U/mg. GST was measured and it was found to be significantly increased in CHA of 582.99 U/mg compared with other commercially available products. The Ascorbic acid Peroxidase activity was higher in Keradix of about 725.31 U/mg APX. The S...
Genetic variations, if any, in Musa accuminata exposed to Humic acid ; Coal extracted Humic acid ... more Genetic variations, if any, in Musa accuminata exposed to Humic acid ; Coal extracted Humic acid (CHA), Biochar Humic acid (BHA) and Commercially available Keradix (KHA) was evaluated using Random amplified polymorphic DNA (RAPD), Simple Sequence Repeat (SSR) and inter simple sequence repeat (ISSR). In this study tissue culture of Musa accuminata enhanced with various concentrations ranging from 0.1 – 0.5% of Humic acid was carried out in MS medium. The isolated DNA amplified with 2 ISSR, 2 SSR and 2 RAPD primers. The PCR based markers such as RAPD and ISSR were useful techniques for detection of variation atgenetic level. The present protocol used for large scale production of Humic acid enhanced Musa accuminata produce genetically stable plants and was proved that it can be used without any problem.
Humic Acid is the most important constituent of a completely decomposed organic matter. Leonardit... more Humic Acid is the most important constituent of a completely decomposed organic matter. Leonardite is found to be the best source for Humic Acid and it is extracted by using alkali solution. Humic Acid scientifically proves to be a free radical scavenger. The study determines that Humic Acid (HA) is used to enhance stress tolerance in plant tissue culture. The induction of the antioxidant system [polyphenol, Catalase (CAT), Superoxide dismutase (SOD),Ascorbate peroxidase (APX) and Glutathione S Transferase (GST)] and also the elevation of the content of the enzymes show that the noted changes provoked the treatment of Humic Acid to Musa accuminata. Humic acid exposed plants (GWO4 –HU) were found to exhibit only 55 µg of GAE g/dw while native plant (GWO4 – NA) exhibited 147.5 µg of GAE g/dw. The increase of polyphenols in the native may be due to the exposure of roots to the heavy metals that are present in activated charcoal used in rooting medium or due to metal ions in water used....
Though vanadium complexes mimic the action of insulin, owing to their toxicity, research is still... more Though vanadium complexes mimic the action of insulin, owing to their toxicity, research is still in progress for a new vanadium complex with maximum efficacy at low concentration and without any side effects. A novel macrocyclic binuclear oxovanadium complex was synthesized, its composition and structure were confirmed by spectral studies and its efficacy was studied in streptozotocin-induced diabetic rats over a period of 30 days. The oral administration of the complex normalizes the blood glucose level in the diabetic rats and also maintains normoglycemia after a glucose load. The biochemical studies revealed that the complex is not toxic to the system. The nontoxic nature of this complex may be due to the presence of the vanadyl ions in an intact form. The study highlights the nontoxic and hypoglycemic effects of the new macrocyclic binuclear oxovanadium complex.
Medicinal herbs used in indigenous medicines for the management of diabetes mellitus contain both... more Medicinal herbs used in indigenous medicines for the management of diabetes mellitus contain both organic and inorganic constituents. Some of these inorganic trace elements possess antidiabetic properties, which accounts for the activity of medicinal herbs. The aim of this study was to analyze the inorganic trace elements present in Eugenia jambolana seeds and to evaluate the hypoglycemic activity of the inorganic part of E. jambolana seeds on streptozotocin-induced diabetes. The seeds of E. jambolana seeds were reduced to ash and the inorganic elements present were assayed. The hypoglycemic efficacy of the inorganic part was tested by the glucose tolerance test on streptozotocin-induced diabetes. Elements such as zinc, chromium, vanadium, potassium, and sodium, possessing hypoglycemic activity, were present in the seed. The E. jambolana seed ash-treated diabetic rats exhibited normoglycemia and better glucose tolerance. The conclusion that the inorganic constituents might play a important role in the antidiabetic nature E. jambolana seeds was reached.
Uploads
Papers by Sathish Sekar