Papers by Andrei Chernyshev
Cytometry Part A, May 22, 2023
International Journal of Energetic Materials and Chemical Propulsion, 2007
Cytometry Part A, 2017
Whereas modern automated blood cell analyzers measure the volume of individual red blood cells (R... more Whereas modern automated blood cell analyzers measure the volume of individual red blood cells (RBCs), leading to four RBC indices (mean corpuscular volume, MCV; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; red cell distribution width, and RDW), the RBC shape has not been assessed by clinical screening tools. We applied the scanning flow cytometer (SFC) for complete characterization of intact RBC morphology in terms of diameter, maximal and minimal thicknesses, volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration, and content. The above‐mentioned individual RBC characteristics were measured without fluorescent markers and other chemicals by a SFC equipped only with 660 nm laser for RBC illumination and single detector for measurement of angle‐resolved light scattering. The distributions over all RBC characteristics were constructed and processed statistically to form the novel 31 RBC indices for 22 donor sample...
Journal of Immunological Methods, 2015
Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, Proceedings Of, 1995
A novel type of Scanning Flow Cytometer (SFC) has been developed for improving particle analysis ... more A novel type of Scanning Flow Cytometer (SFC) has been developed for improving particle analysis from light scattering data. The use of the SFC and the Flying Light Scattering Indicatrix (FLSI) method allows absolute measurement of the size and refractive index of spherical particles. The separation efficiency of the single particle analysis using FLSI method is verified measuring the size distribution of standard latex particles. It is shown that particles differed in size more than 1.2 micrometers can be separated by the FLSI method to different classes. In the second part of this paper the feasibility of a Pulsed Laser Flow Cytometer (PLFC) equipped by a pulsed nitrogen laser is tested for fluorometric analysis of human lymphocytes stained with FITC-labeled monoclonal antibodies. In addition the feasibility for the fluorescence measurement in time-resolved regime of the PLFC is analyzed from measurement of photon collection efficiency of scanning optical system.
Journal of Theoretical Biology, 2016
Physical Chemistry Chemical Physics, 2007
Measurement of scattering properties of individual particles with a scanning flow cytometer
Background: Detection of human immunotoxicity is relevant to predictive and regulatory toxicology... more Background: Detection of human immunotoxicity is relevant to predictive and regulatory toxicology but is usually complex, while nodal steps may provide simple and suitable endpoints. NF-kB activation is both a promising therapeutic target and an important immunotoxicity endpoint. Conventional methods employed to determine nuclear translocation of NF-kB lack statistical robustness (microscopy) or the ability to discern heterogeneity within the sampled populations (Western blotting and gel shift assays). Previously, we developed an in vitro assay of immunotoxicity based on quantitative measurement by Multispectral Image-in-Flow Cytometry (MsIFC) of NF-kB translocation in a monocytic cell line. MsIFC combines the high image content information of microscopy with the high throughput and multiparametric analysis of flow cytometry which overcomes the aforementioned limitations of conventional assays. In this study, we have adapted and validated this assay for human peripheral whole-blood ...
Journal of Quantitative Spectroscopy and Radiative Transfer, 2017
Journal of Applied Spectroscopy, 1996
A technique for in situ characterization of the RESS process (Rapid Expansion of Supercrit. Solns... more A technique for in situ characterization of the RESS process (Rapid Expansion of Supercrit. Solns.) was developed and applied to RDX nano-particles formation. The technique is based on the combination of laser scattering and time resolved imaging. An excimer laser (248 nm) was used in combination with a fast gated ICCD camera. Particle size distribution functions downstream the RESS jet were obtained for neat CO2 as well as for supercrit. solns. of RDX in CO2. Plausible particle growth mechanisms are discussed. [on SciFinder(R)]
Tsitologiia, 2012
We suggest a new theoretical method of the flow cytometry DNA histograms and apply it for Drosoph... more We suggest a new theoretical method of the flow cytometry DNA histograms and apply it for Drosophila melanogaster imaginal discs cells. The model gives a possibility to determine the proportions of cells in G1, G2 (M) and S cell cycle phases. We show that the precision of G1 and G2 (M) DNA content measurements is limited by the precision of device zero signal arrangement. The usage of calculated device zero and dividing cells as the DNA content standards may improve the precision of DNA content measurements. We also compared the precisions of different DNA content methods and draw the conclusion that the current precisions of different methods are similar and lie within 2-6% interval.
Controlling Tissue Optical Properties: Applications in Clinical Study, 2000
ABSTRACT
Doklady Physical Chemistry
Chemical Physics Letters, 1993
ABSTRACT
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Papers by Andrei Chernyshev