Abstract. Air-water gas exchange is essential to understanding and quantifying many biogeochemical processes in streams and rivers, including greenhouse gas emissions and metabolism. Gas exchange depends on two factors, which are often quantified separately: 1) the air-water concentration gradient of the gas and 2) the gas exchange velocity. There are fewer measurements of gas exchange velocity compared to concentrations in streams and rivers, which limits accurate characterization of air-water gas exchange (i.e., flux rates). The National Ecological Observatory Network (NEON) conducts SF₆ gas-loss experiments in 22 of their 24 wadeable streams using standardized methods across all experiments and sites, and publishes raw concentration data from these experiments on the NEON data portal. NEON also conducts NaCl injections that can be used to characterize hydraulic geometry at all 24 wadeable streams. These NaCl injections are conducted both as part of the gas-loss experiments and separately. Here, we use these data to estimate gas exchange and water velocity using the reaRates R package. The dataset presented includes estimates of hydraulic parameters, cleaned raw concentration SF₆ tracer-gas data (including removing outliers and failed experiments), estimated SF₆ gas loss rates, normalized gas exchange velocities (k₆₀₀, m d⁻¹) and normalized depth-dependent gas exchange rates (K₆₀₀, d⁻¹). This dataset provides one of the largest compilations of gas loss experiments (n = 339) in streams to date. This dataset is unique in that it contains gas exchange estimates from repeated experiments in geographically diverse streams across a range of discharges. In addition, this dataset contains information on the hydraulic geometry of all 24 NEON wadeable streams, which will support future research using NEON aquatic data. This dataset is a valuable resource that can be used to explore both within- and across-reach variability in the hydraulic geometry and gas exchange velocity in streams.