1990) and 16S rDNA was amplified by using the universal
eubacterial primers such as forward primer, 5'-AGAGTTTGATCCTGGCTCAG-3' and reverse primer, 5'-ACGGCTACCTTGTTACGACTT-3' (Allen et al.
16S ribosomal RNA genes were amplified using universal
eubacterial primers, and PCR products were cloned and sequenced.
Amplification of the 16S rRNA gene was carried out with Ilustra[TM] PureTaq Ready-To-Go PCR beads (GE Healthcare, UK) using the universal
eubacterial primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'T-ACGGCT ACCTT GTT ACGACTT-3 ').
The 16S rRNA universal
eubacterial primers 28F (5'-GAGTTTGATCITGGCTCAG) and 519R (5,-GWATTACCGCGGCKGCTG) were used to amplify approximately 500 bp of the variable regions V1-V3.
larvae, because these primer pair was universal
eubacterial primer that designed to classify related bacteria depending on sequencing results.
(1999) Purification, characterization, and cloning of a
eubacterial 3-hydroxy-3-methylglutaryl coenzyme A reductase, a key enzyme involved in biosynthesis of terpenoids.
(2) Positive control: university
eubacterial probe EU338.
Polymerase chain reaction (PCR) amplification of the bacterial 16S gene was performed using the universal
eubacterial primer set 27f-1387r (27f: 5'-AGA GTT TGA TCM TGG CTC AG-3') and 1387r (5'-GGG CGG WGT GTA CAA GGC-3') (Marchesi et al.
Most of these cells are archaeal rather than
eubacterial. If similar results are found elsewhere, this means that archaea are the most abundant cells in the ocean system and rival eubacteria in total numbers on earth.
DNA and c-DNA were amplified in a PCR thermocycler (Bio-Rad Laboratories, CA, USA) with the following primer pairs (MWG-Biotech AG, Germany): (i) 968F1401R
eubacterial universal primers to amplify a ~500 bp region of the 16S rDNA gene (Nubel et al.
Closest sequence matches were to the
eubacterial genera Mycoplasma, Salinispora, Frankia, and Pelodictyon.
Following are individual chapters on specific genus and species, and coverage of other relevant topics including introductions to parasites, yeasts, and virology, and a survey of clinical, commercial, and research-model
eubacterial species.