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{{about|the biochemical [[buffering agent]]|information about other types of mops|Mops (disambiguation)}}
{{About|the biochemical buffering agent|information about other types of mops|Mops (disambiguation){{!}}Mops}}
{{chembox
{{Chembox
| Verifiedfields = changed
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| verifiedrevid = 407780760
| Watchedfields = changed
|ImageFile=MOPS.png
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| verifiedrevid = 442344970
|ImageSize=200px
| ImageFile = Mops is.svg
|IUPACName=3-morpholinopropane-1-sulfonic acid
| PIN = 3-(Morpholin-4-yl)propane-1-sulfonic acid
|OtherNames=3-(N-morpholino)propanesulfonic acid
| OtherNames = 3-(''N''-Morpholino)propanesulfonic acid,<br />3-Morpholinopropanesulfonic acid,<br />3-''N''-Morpholino propansulfonic acid,<br />4-Morpholinepropanesulfonic acid
|Section1= {{Chembox Identifiers
| Section1 = {{Chembox Identifiers
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| CASNo=1132-61-2
| CASNo_Ref = {{cascite|correct|??}}
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| PubChem=70807
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| CASNo = 1132-61-2
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| SMILES=C1COCCN1CCCS(=O)(=O)O
| UNII_Ref = {{fdacite|correct|FDA}}
| UNII = 273BP63NV3
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| PubChem = 70807
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| SMILES = C1COCCN1CCCS(=O)(=O)O
| PubChem2 = 2723950
| ChemSpiderID_Ref = {{chemspidercite|changed|chemspider}}
| ChemSpiderID = 63972
| InChI = 1/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)
| InChIKey = DVLFYONBTKHTER-UHFFFAOYAN
| StdInChI_Ref = {{stdinchicite|changed|chemspider}}
| StdInChI = 1S/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)
| StdInChIKey_Ref = {{stdinchicite|changed|chemspider}}
| StdInChIKey = DVLFYONBTKHTER-UHFFFAOYSA-N
}}
}}
|Section2= {{Chembox Properties
| Section2 = {{Chembox Properties
| C=7|H=15|N=1|O=4|S=1
| Formula=C<sub>7</sub>H<sub>15</sub>NO<sub>4</sub>S
| Appearance =
| MolarMass=209.2633
| Appearance=
| Density =
| Density=
| MeltingPt =
| MeltingPt=
| BoilingPt =
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| Solubility =
| BoilingPt=
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| Solubility=
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|Section3= {{Chembox Hazards
| Section3 = {{Chembox Hazards
| GHSPictograms = {{GHS07}}
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| MainHazards=
| GHSSignalWord = Warning
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| FlashPt=
| HPhrases = {{H-phrases|315|319|335}}
| Autoignition=
| PPhrases = {{P-phrases|261|264|271|280|302+352|304+340|305+351+338|312|321|332+313|337+313|362|403+233|405|501}}
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| MainHazards =
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| FlashPt =
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| ExternalSDS =
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}}
}}
}}

'''MOPS''' is the common name for the compound '''3-(N-morpholino)propanesulfonic acid''', a [[Buffer solution|buffer]] introduced by Good et al. in the 1960s. It is a structural analog to [[MES (buffer)|MES]].<ref>{{cite journal|doi=10.1021/bi00866a011|title=Hydrogen Ion Buffers for Biological Research|year=1966|last1=Good|first1=Norman E.|last2=Winget|first2=G. Douglas|last3=Winter|first3=Wilhelmina|last4=Connolly|first4=Thomas N.|last5=Izawa|first5=Seikichi|last6=Singh|first6=Raizada M. M.|journal=Biochemistry|volume=5|pages=467–77|pmid=5942950|issue=2}}</ref> Its chemical structure contains a [[morpholine]] ring. [[HEPES]] is a similar [[pH]] buffering compound that contains a [[piperazine]] ring. With a [[pKa|pK<sub>a</sub>]] of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
'''MOPS''' ('''3-(''N''-morpholino)propanesulfonic acid''') is a [[buffer solution|buffer]] introduced in the 1960s, one of the twenty [[Good's buffers]]. It is a structural analog to [[MES (buffer)|MES]],<ref>{{cite journal|doi=10.1021/bi00866a011|title=Hydrogen Ion Buffers for Biological Research|year=1966|last1=Good|first1=Norman E.|last2=Winget|first2=G. Douglas|last3=Winter|first3=Wilhelmina|last4=Connolly|first4=Thomas N.|last5=Izawa|first5=Seikichi|last6=Singh|first6=Raizada M. M.|journal=Biochemistry|volume=5|pages=467–77|pmid=5942950|issue=2}}</ref> and like MES, its structure contains a [[morpholine]] ring. [[HEPES]] is a similar [[pH]] buffering compound that contains a [[piperazine]] ring. With a [[pKa|p''K''<sub>a</sub>]] of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.


==Applications==
==Applications==
MOPS is frequently used as a [[buffering agent]] in [[biology]] and [[biochemistry]]. It has been tested and recommended for [[polyacrylamide gel electrophoresis]].<ref>{{cite journal|doi=10.1016/0003-2697(81)90178-0|pmid=6278979|title=A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH|first2=ME|year=1981|last2=Hodes|last1=Thomas|first1=J|journal=Analytical Biochemistry|volume=118|issue=1|pages=194–6}}</ref> Usage above 20 mM in mammalian [[cell culture]] work is not recommended.<ref>{{cite journal|doi=10.1126/science.174.4008.500|pmid=5110427|title=Buffer Combinations for Mammalian Cell Culture|year=1971|last1=Eagle|first1=H.|journal=Science|volume=174|issue=4008|pages=500–3}}</ref>
MOPS is frequently used as a [[buffering agent]] in [[biology]] and [[biochemistry]]. It has been tested and recommended for [[polyacrylamide gel electrophoresis]].<ref>{{cite journal|doi=10.1016/0003-2697(81)90178-0|pmid=6278979|title=A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH|first2=ME|year=1981|last2=Hodes|last1=Thomas|first1=J|journal=Analytical Biochemistry|volume=118|issue=1|pages=194–6}}</ref> Usage above 20 mM in mammalian [[cell culture]] work is not recommended.<ref>{{cite journal|doi=10.1126/science.174.4008.500|pmid=5110427|title=Buffer Combinations for Mammalian Cell Culture|year=1971|last1=Eagle|first1=H.|journal=Science|volume=174|issue=4008|pages=500–3|bibcode=1971Sci...174..500E|s2cid=29876583}}</ref> MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.<ref>{{cite web |url=https://bostonbioproducts.com/products/mops-buffer-1-m-ph-74-bbm-74}}</ref>


==See also==
==See also==
⚫
*[[CAPS (buffer)|CAPS]]
{{colbegin|3}}
*[[MES (buffer)|MES]]
*[[HEPPS (molecule)|HEPPS]]
*[[HEPES]]
*[[CAPSO]]
⚫
*[[HEPPS (buffer)|HEPPS]]
*[[Tris]]
*[[Tris]]
*[[Buffer_solution#Common_buffer_compounds_used_in_biology|Common buffer compounds used in biology]]
*[[Buffer_solution#Common_buffer_compounds_used_in_biology|Common buffer compounds used in biology]]
{{colend}}


==References==
==References==
{{reflist|2}}
{{reflist}}


==External links==
==External links==

*[http://hazard.com/msds/mf/baker/baker/files/m8014.htm Safety information]
*[http://www.sigmaaldrich.com/life-science/core-bioreagents/biological-buffers/learning-center/buffer-calculator.html Sigma Aldrich Buffer Calculator] - Useful tool to calculate weight, volume, or concentration from molecular weight.
*[http://www.sigmaaldrich.com/life-science/core-bioreagents/biological-buffers/learning-center/buffer-calculator.html Sigma Aldrich Buffer Calculator] - Useful tool to calculate weight, volume, or concentration from molecular weight.
*[http://openwetware.org/index.php?title=MOPS Recipe for MOPS buffer on OpenWetWare]
*[http://openwetware.org/index.php?title=MOPS Recipe for MOPS buffer on OpenWetWare]


[[Category:Buffers]]
[[Category:Buffer solutions]]
[[Category:Sulfonic acids]]
[[Category:Sulfonic acids]]
[[Category:Morpholines]]
[[Category:4-Morpholinyl compounds]]

{{biochem-stub}}

[[ru:3-Морфолинопропансульфоновая кислота]]
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