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Genetic classification of Cryptosporidium isolates from humans and calves in Slovenia

Published online by Cambridge University Press:  29 July 2008

B. SOBA  and
J. LOGAR
B. SOBA*
Affiliation:
Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloska 4, 1000 Ljubljana, Slovenia
J. LOGAR
Affiliation:
Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloska 4, 1000 Ljubljana, Slovenia
*
*Corresponding author: Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloska 4, 1000 Ljubljana, Slovenia. Tel: +386 1 543 74 71. Fax:+386 1 543 74 01. E-mail: barbara.soba@mf.uni-lj.si
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Summary

To assess the importance of cattle as a source of human cryptosporidial infections in Slovenia, Cryptosporidium isolates from calves and humans with cryptosporidiosis were characterized genetically by direct DNA sequencing, targeting a variable region of the 60 kDa glycoprotein (gp60) gene. In total, 15 genetic variants, designated ‘subtypes’, were identified, of which 7 were novel. In humans, C. hominis Ia (subtype IaA17R3) and Ib (IbA10G2) and Cryptosporidium parvum IIa (IIaA9G1R1, IIaA11G2R1, IIaA13R1, IIaA14G1R1, IIaA15G1R1, IIaA15G2R1, IIaA16G1R1, IIaA17G1R1 and IIaA19G1R1), IIc (IIcA5G3), and IIl (IIlA16R2) were recorded; this is the first record of the latter subtype in humans. In cattle, C. parvum IIa (IIaA13R1, IIaA15G2R1, IIaA16R1 and IIaA16G1R1) and IIl (IIlA16R2 and IIlA18R2) were recorded. Of the 15 subtypes identified, subtypes of C. parvum IIa were the most frequently encountered (>90%) in both humans and calves. The present findings suggest that zoonotic transmission plays an important role in sporadic human cryptosporidiosis in Slovenia.

Keywords

Cryptosporidiumsubtypeshumanscalves60 kDa glycoprotein gene sequencesSlovenia
Type
Original Articles
Information
Parasitology , Volume 135 , Issue 11 , September 2008 , pp. 1263 - 1270
Copyright
Copyright © 2008 Cambridge University Press

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