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Authors: Juan A. Serrano 1 ; Pablo Pérez 1 ; Andrés Maldonado 1 ; María Martín 2 ; Alberto Olmo 1 ; Paula Daza 2 ; Gloria Huertas 1 and Alberto Yúfera 1

Affiliations: 1 Instituto de Microelectrónica de Sevilla (IMSE), Universidad de Sevilla, Spain ; 2 Facultad de Biología, Universidad de Sevilla, Spain

Keyword(s): Biomedical Circuits, Impedance Spectroscopy, Bioimpedance, Electrode-model, Oscillation based Test Circuits (OBT), ECIS.

Abstract: This paper presents the fitting process followed to adjust the parameters of the electrical model associated to a cell-electrode system in Electrical Cell-substrate Impedance Spectroscopy (ECIS) technique, to the experimental results from cell-culture assays. A new parameter matching procedure is proposed, under the basis of both, mismatching between electrodes and time-evolution observed in the system response, as consequence of electrode fabrication processes and electrochemical performance of electrode-solution interface, respectively. The obtained results agree with experimental performance, and enable the evaluation of the cell number in a culture, by using the electrical measurements observed at the oscillation parameters in the test circuits employed.

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Paper citation in several formats:
Serrano, J. A., Pérez, P., Maldonado, A., Martín, M., Olmo, A., Daza, P., Huertas, G. and Yúfera, A. (2018). Practical Characterization of Cell-Electrode Electrical Models in Bio-Impedance Assays. In Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2018) - BIODEVICES; ISBN 978-989-758-277-6; ISSN 2184-4305, SciTePress, pages 100-108. DOI: 10.5220/0006712601000108

@conference{biodevices18,
author={Juan A. Serrano and Pablo Pérez and Andrés Maldonado and María Martín and Alberto Olmo and Paula Daza and Gloria Huertas and Alberto Yúfera},
title={Practical Characterization of Cell-Electrode Electrical Models in Bio-Impedance Assays},
booktitle={Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2018) - BIODEVICES},
year={2018},
pages={100-108},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0006712601000108},
isbn={978-989-758-277-6},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the 11th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2018) - BIODEVICES
TI - Practical Characterization of Cell-Electrode Electrical Models in Bio-Impedance Assays
SN - 978-989-758-277-6
IS - 2184-4305
AU - Serrano, J.
AU - Pérez, P.
AU - Maldonado, A.
AU - Martín, M.
AU - Olmo, A.
AU - Daza, P.
AU - Huertas, G.
AU - Yúfera, A.
PY - 2018
SP - 100
EP - 108
DO - 10.5220/0006712601000108
PB - SciTePress

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