Porcine TRIM21 Enhances Porcine Circovirus 2 Infection and Host Immune Responses, But Inhibits Apoptosis of PCV2-Infected Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Virus
2.2. Drugs and Antibodies
2.3. Plasmid Construction
2.4. RNA/DNA Extraction and Amplification
2.5. Construction of Overexpression and Knocking out Cells
2.6. MTS Assay
2.7. Western Blot (WB)
2.8. Indirect Immunofluorescence Assay (IFA)
2.9. ELISA
2.10. Caspase Activity Assay
2.11. Statistical Analysis
3. Results
3.1. PCV2 Infection Modulated the Levels of Porcine TRIM21
3.2. Porcine TRIM21 Is Positively Associated with the PCV2 Infection
3.3. Porcine TRIM21 Decreases Cellular Apoptosis in PCV2-Infected Cells
3.4. Porcine TRIM21 Enhances Interferons and Proinflammatory Factors in PCV2-Infected Cells
3.5. Porcine TRIM21 Increases Porcine MNDAL Expression
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yang, L.; Liu, X.; Zhang, L.; Li, X.; Zhang, X.; Niu, G.; Ji, W.; Chen, S.; Ouyang, H.; Ren, L. Porcine TRIM21 Enhances Porcine Circovirus 2 Infection and Host Immune Responses, But Inhibits Apoptosis of PCV2-Infected Cells. Viruses 2022, 14, 156. https://doi.org/10.3390/v14010156
Yang L, Liu X, Zhang L, Li X, Zhang X, Niu G, Ji W, Chen S, Ouyang H, Ren L. Porcine TRIM21 Enhances Porcine Circovirus 2 Infection and Host Immune Responses, But Inhibits Apoptosis of PCV2-Infected Cells. Viruses. 2022; 14(1):156. https://doi.org/10.3390/v14010156
Chicago/Turabian StyleYang, Lin, Xiaohua Liu, Liying Zhang, Xue Li, Xinwei Zhang, Guyu Niu, Weilong Ji, Si Chen, Hongsheng Ouyang, and Linzhu Ren. 2022. "Porcine TRIM21 Enhances Porcine Circovirus 2 Infection and Host Immune Responses, But Inhibits Apoptosis of PCV2-Infected Cells" Viruses 14, no. 1: 156. https://doi.org/10.3390/v14010156