Nanopore-Based Direct RNA-Sequencing Reveals a High-Resolution Transcriptional Landscape of Porcine Reproductive and Respiratory Syndrome Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Preparation and Total RNA Extraction
2.2. Library Preparation and Sequencing
2.3. Genome-Wide PRRSV Phylogenetic Analysis
2.4. Data Analysis
2.5. Identification of 5mC Methylation
3. Results
3.1. The Prevalent Status and Genetic Diversity of PRRSV-2 in China
3.2. NADC30-Like PRRSV and HP-PRRSV Induce Different Transcriptional Activity during Infection in Susceptible PAM Cells
3.3. Analysis of Alternative Splicing Events during Transcription
3.4. Identification of TRS-B Sites in PRRSV Genome
3.5. Gene Predictions and Annotations
3.6. Revealing m5C Sites in gRNA and sg mRNAs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhang, R.; Wang, P.; Ma, X.; Wu, Y.; Luo, C.; Qiu, L.; Zeshan, B.; Yang, Z.; Zhou, Y.; Wang, X. Nanopore-Based Direct RNA-Sequencing Reveals a High-Resolution Transcriptional Landscape of Porcine Reproductive and Respiratory Syndrome Virus. Viruses 2021, 13, 2531. https://doi.org/10.3390/v13122531
Zhang R, Wang P, Ma X, Wu Y, Luo C, Qiu L, Zeshan B, Yang Z, Zhou Y, Wang X. Nanopore-Based Direct RNA-Sequencing Reveals a High-Resolution Transcriptional Landscape of Porcine Reproductive and Respiratory Syndrome Virus. Viruses. 2021; 13(12):2531. https://doi.org/10.3390/v13122531
Chicago/Turabian StyleZhang, Riteng, Peixin Wang, Xin Ma, Yifan Wu, Chen Luo, Li Qiu, Basit Zeshan, Zengqi Yang, Yefei Zhou, and Xinglong Wang. 2021. "Nanopore-Based Direct RNA-Sequencing Reveals a High-Resolution Transcriptional Landscape of Porcine Reproductive and Respiratory Syndrome Virus" Viruses 13, no. 12: 2531. https://doi.org/10.3390/v13122531