A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Virus Inoculation
2.2. Construction of NbCXE Over-Expression and TRV Silencing Vectors
2.3. Transient Over-Expression of NbCXE and TRV-Based Gene Silencing of NbCXE
2.4. Total RNA Isolation and Western Blot
2.5. qRT-PCR Analysis
2.6. Yeast Two-Hybrid Assay
3. Results
3.1. Identification of the New Resistance-Related Gene NbCXE in N. benthamiana Plants
3.2. Over-Expression of NbCXE Inhibited TMV Accumulation in N. benthamiana Plants
3.3. Silencing of NbCXE Enhanced TMV Accumulation
3.4. Interaction of NbCXE with TMV CP
3.5. Expression of Host Defense-Related Genes Increased in Transient NbCXE-Overexpressed Plants but Decreased in NbCXE-Silenced N. benthamiana Plants
4. Discussion
4.1. NbCXE Encoded a Resistance-Related Protein
4.2. NbCXE Interacted with TMV CP
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Guo, S.; Wong, S.-M. A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants. Viruses 2020, 12, 195. https://doi.org/10.3390/v12020195
Guo S, Wong S-M. A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants. Viruses. 2020; 12(2):195. https://doi.org/10.3390/v12020195
Chicago/Turabian StyleGuo, Song, and Sek-Man Wong. 2020. "A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants" Viruses 12, no. 2: 195. https://doi.org/10.3390/v12020195