New Insights into Curcumin- and Resveratrol-Mediated Anti-Cancer Effects
Abstract
:1. Introduction
2. Results
2.1. Curcumin, Resveratrol and Their Combination Impair TUBO and SALTO Cell Survival
2.2. Curcumin and Resveratrol Differently Affect Autophagy in Both Cell Lines
2.3. Curcumin and Resveratrol Have a Different Impact on PI3K/AKT/mTOR Activation
2.4. Curcumin and Resveratrol Dysregulate ROS in TUBO and SALTO Cell Lines
2.5. Curcumin/Resveratrol Combination Upregulates the PERK/eIF2alpha/CHOP Axis of UPR in Both TUBO and SALTO Cells
3. Discussion
4. Materials and Methods
4.1. Cell Cultures and Treatments
4.2. Trypan Blue Exclusion Assay
4.3. MTT Cell Proliferation Assay
4.4. Measurement of Intracellular Reactive Oxygen Species [50] Production
4.5. Western Blot Analysis
4.6. Antibodies
4.7. Densitometric Analysis
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Arena, A.; Romeo, M.A.; Benedetti, R.; Masuelli, L.; Bei, R.; Gilardini Montani, M.S.; Cirone, M. New Insights into Curcumin- and Resveratrol-Mediated Anti-Cancer Effects. Pharmaceuticals 2021, 14, 1068. https://doi.org/10.3390/ph14111068
Arena A, Romeo MA, Benedetti R, Masuelli L, Bei R, Gilardini Montani MS, Cirone M. New Insights into Curcumin- and Resveratrol-Mediated Anti-Cancer Effects. Pharmaceuticals. 2021; 14(11):1068. https://doi.org/10.3390/ph14111068
Chicago/Turabian StyleArena, Andrea, Maria Anele Romeo, Rossella Benedetti, Laura Masuelli, Roberto Bei, Maria Saveria Gilardini Montani, and Mara Cirone. 2021. "New Insights into Curcumin- and Resveratrol-Mediated Anti-Cancer Effects" Pharmaceuticals 14, no. 11: 1068. https://doi.org/10.3390/ph14111068