Pathogenicity Differentiation of Fusarium spp. Causing Fusarium Basal Rot and Wilt Disease in Allium spp.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strains and Plant Materials
2.2. DNA Isolation, Polymerase Chain Reaction (PCR), and Sequencing
2.3. Phylogenetic Analysis
2.4. Onion Bulb Inoculation Test
2.5. Welsh Onion Seedling Inoculation Test
2.6. RNA Extraction and Reverse Transcriptaion (RT)-PCR
2.7. Statistical Analyses
3. Results
3.1. Verification of the Taxonomic Position of Foc Strains
3.2. Variation of SIX Gene Possession and Gene Expression Profiles
3.3. Inoculation Tests in Onion Bulbs and Welsh Onion Seedlings
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SIX Gene | Accession Number a | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Strain | Host | Origin | 2 | 3 | 5 | 7 | 9 | 10 | 12 | 14 | cmdA | rpb2 | tef1 |
AC145 | Onion | The Netherlands | − | + | + | + | + | + | + | + | LC795549 | LC795537 | LC795525 |
AP117 | Onion | Australia | − | + | + | + | + | + | + | + | LC795540 | LC795527 | LC795524 |
Ru-13 | Onion | Russia | − | + | + | + | + | + | + | + | LC795541 | LC795526 | LC795523 |
TA | Onion | Japan | − | + | + | + | + | + | + | + | LC795548 | LC795536 | AB938076 |
AF67 | Welsh onion | Japan | − | − | − | − | − | − | − | − | LC795543 | LC795532 | AB938030 |
AF74 | Welsh onion | Japan | + | − | − | − | − | − | − | − | LC795544 | LC795530 | AB938032 |
AF97 | Welsh onion | Japan | − | − | − | − | − | − | − | − | LC795546 | LC795533 | AB938043 |
AF113 | Welsh onion | Japan | − | − | − | − | + | − | − | − | LC795547 | LC795528 | AB938047 |
AF17 | Welsh onion | Japan | + | − | − | − | + | − | − | − | LC795542 | LC795529 | AB938024 |
AF22 | Welsh onion | Japan | + | − | − | − | + | − | − | − | LC795538 | LC795531 | AB938025 |
AF90 | Welsh onion | Japan | + | − | − | − | + | − | − | − | LC795545 | LC795534 | AB938037 |
AF94 | Welsh onion | Japan | + | − | − | − | + | − | − | − | LC795539 | LC795535 | AB938040 |
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Sakane, K.; Ueno, T.; Shigyo, M.; Sasaki, K.; Ito, S.-i. Pathogenicity Differentiation of Fusarium spp. Causing Fusarium Basal Rot and Wilt Disease in Allium spp. Pathogens 2024, 13, 591. https://doi.org/10.3390/pathogens13070591
Sakane K, Ueno T, Shigyo M, Sasaki K, Ito S-i. Pathogenicity Differentiation of Fusarium spp. Causing Fusarium Basal Rot and Wilt Disease in Allium spp. Pathogens. 2024; 13(7):591. https://doi.org/10.3390/pathogens13070591
Chicago/Turabian StyleSakane, Kosei, Takashi Ueno, Masayoshi Shigyo, Kazunori Sasaki, and Shin-ichi Ito. 2024. "Pathogenicity Differentiation of Fusarium spp. Causing Fusarium Basal Rot and Wilt Disease in Allium spp." Pathogens 13, no. 7: 591. https://doi.org/10.3390/pathogens13070591