Metabolomic Comparison of Guava (Psidium guajava L.) Leaf Extracts Fermented by Limosilactobacillus fermentum and Lactiplantibacillus plantarum and Their Antioxidant and Antiglycation Activities
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Lactobacillus Strains and Culture Conditions
2.3. Sample Preparation and Extraction
2.4. GC-TOF-MS Analysis
2.5. UHPLC–Orbitrap–MS/MS Analysis
2.6. Data Processing and Statistical Analysis
2.7. Determination of the Antioxidant Activities and Antiglycation Activities
3. Results
3.1. Metabolic Profiling of the Guava Leaf Groups (0%, 0.5%, and 2%) Fermented by L. fermentum and L. plantarum
3.2. Metabolic Pathway Comparison of L. fermentum and L. plantarum Fermentation in the Guava Leaf Extract Groups (0%, 0.5%, and 2%)
3.3. Bioconversion of the Guava-Derived Metabolites Fermented by L. fermentum and L. plantarum
3.4. Bioactivity in the Groups Supplemented with the Guava Leaf Extracts (0%, 0.5%, and 2%) and Fermented by L. fermentum and L. plantarum
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Substrate | Inoculation | Abb a | Symbol |
---|---|---|---|
MRS | - | NFG-0 | |
(0% group) | Limosilactobacillus fermentum KCTC15072BP | LFG-0 | |
Lactiplantibacillus plantarum KGMB00831 | LPG-0 | ||
MRS with 0.5% (w/v) guava leaf extract | - | NFG-0.5 | |
(0.5% group) | Limosilactobacillus fermentum KCTC15072BP | LFG-0.5 | |
Lactiplantibacillus plantarum KGMB00831 | LPG-0.5 | ||
MRS with 2% (w/v) guava leaf extract | - | NFG-2 | |
(2% group) | Limosilactobacillus fermentum KCTC15072BP | LPG-2 | |
Lactiplantibacillus plantarum KGMB00831 | LFG-2 |
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Jun, B.-G.; Kim, S.-H.; Kim, S.-H.; Hong, S.-M.; Lee, H.; Lim, Y.; Kim, S.-Y.; Lee, C.-H. Metabolomic Comparison of Guava (Psidium guajava L.) Leaf Extracts Fermented by Limosilactobacillus fermentum and Lactiplantibacillus plantarum and Their Antioxidant and Antiglycation Activities. Nutrients 2024, 16, 841. https://doi.org/10.3390/nu16060841
Jun B-G, Kim S-H, Kim S-H, Hong S-M, Lee H, Lim Y, Kim S-Y, Lee C-H. Metabolomic Comparison of Guava (Psidium guajava L.) Leaf Extracts Fermented by Limosilactobacillus fermentum and Lactiplantibacillus plantarum and Their Antioxidant and Antiglycation Activities. Nutrients. 2024; 16(6):841. https://doi.org/10.3390/nu16060841
Chicago/Turabian StyleJun, Bo-Gyu, Su-Hyun Kim, Seon-Hyeok Kim, Seong-Min Hong, Heaji Lee, Yunsook Lim, Sun-Yeou Kim, and Choong-Hwan Lee. 2024. "Metabolomic Comparison of Guava (Psidium guajava L.) Leaf Extracts Fermented by Limosilactobacillus fermentum and Lactiplantibacillus plantarum and Their Antioxidant and Antiglycation Activities" Nutrients 16, no. 6: 841. https://doi.org/10.3390/nu16060841